UJI AKTIVITAS ANTIKETOMBE PADA SHAMPO YANG MENGANDUNG MINYAK KEMIRI (Aleuritas Moluccana) DAN JINTAN HITAM (Nigella Sativa) DENGAN METODE DIFUSI SUMURAN

Abstract

Background: Dandruff is commonly caused by the growth of microorganisms, such as Staphylococcus aureus and Staphylococcus epidermidis. One solution to address dandruff is the use of shampoos containing natural active ingredients such as candle nut oil (Aleuritas Moluccana) and black cumin seeds (Nigella sativa), which are known to have antimicrobial effects. This study uses the well diffusion method. Objective: This study aims to test the anti-dandruff activity of shampoo containing candlenut oil (Aleuritas Moluccana) and black cumin seeds (Nigella sativa). Method: This research method uses the well diffusion method. Wells are created on agar media that has been inoculated with test bacteria, and then shampoo is dropped into the wells. After incubation, a zone free of microorganism forms around the well, which is measured to assess the antimicrobial activity of the shampoo. Result: The results of the antidandruff activity test on the shampoo containing candle nut oil (Aleuritas Moluccana) and black cumin seeds (Nigella sativa) against Staphylococcus aureus showed an inhibition zone of 22.33 mm, while against Staphylococcus epidermidis, an inhibition zone of 19.70 mm was observed. Conclusion: Shampoo containing candlenut oil (Aleurites moluccana) and black cumin (Nigella sativa) has anti-dandruff activity against Staphylococcus aureus and Staphylococcus epidermidis.

Keywords: Candle Nut, Black Cumin, Well Diffusion, Staphylococcus aureus, Staphylococcus epidermidis