Potensi Bakteri Tanah Kawasan Terminal dalam Mendegradasi Oli Bekas

Abstract

Environmental pollution caused by used oil is a serious problem due to its toxic hydrocarbon content, which is difficult to break down naturally. This study aims to examine the potential of bacteria isolated from the Amplas Terminal area to degrade used oil. Samples were isolated from three locations contaminated with oil spills and selected based on their ability to grow on mineral media with the addition of used oil as a carbon source. Molecular identification of selected isolates was performed by 16S rRNA gene analysis to determine the type of bacteria. The degradation ability of selected bacteria was measured based on the reduction in oil residue weight and bacterial growth. A total of 23 isolates with different morphologies were successfully isolated, and four isolates (A3, A4, B8, and C4) showed the ability to grow on media enriched with oil. Isolate A4 showed the highest used oil degradation ability (57.06%). Molecular identification results showed that isolate A4 belonged to the species Acinetobacter baumannii. The ability to degrade used oil components was determined through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. GC-MS analysis clarified that most (>90%) of the components of used oil were degraded after 30 days of treatment. The results of this study explain that bacteria isolated from contaminated areas can be used as potential agents for the bioremediation of used oil contamination.