Induksi Tunas Manggis (Garcinia mangostana L.) dengan Pemberian ZPT NAA (Naphtalene Acetic Acid) dan BAP (Benzyl Amino Purine) Secara In Vitro

Abstract

This study aims to determine the effect of the combination of NAA (Napthalene Acetic Acid) and BAP (Benzyl Amino Purine) on the formation of mangosteen buds (Garcinia mangostana L.) In Vitro. This research was conducted at the Laboratory of Alifa Agricultural Research Center (ALIFA-ARC) Medan, from January to May 2025. This study used a completely randomized design (CRD) method with two treatment factors and three replicates. The first factor is NAA concentration consisting of N0 (0 mg/L), N1 (0.5 mg/L) and N2 (1 mg/L). The second factor is the concentration of BAP consisting of B0 (0 mg/L), B1 (3 mg/L) and B2 (6 mg/L). Parameters observed were the percentage of live explants, percentage of contaminated explants, percentage of explants forming buds, time of bud emergence, number of buds, shoot height, number of roots and number of leaves. The results showed that the administration of NAA did not have a significant effect on all observation parameters. The provision of BAP had a significant effect on the parameters of the percentage of explants forming shoots, but did not have a significant effect on the parameters of the percentage of living explants, the percentage of contaminated explants, the time to appear shoots, the number of shoots, shoot height, the number of roots and the number of leaves. The interaction between the treatment combination of NAA and BAP had a significant effect on the parameters of the percentage of living explants and the number of roots, but did not have a significant effect on the parameters of the percentage of contaminated explants, the percentage of explants forming buds, the time to appear buds, the number of buds, the height of the shoots and the number of leaves.