Pengaruh Penambahan Berbagai Jenis Susu (Sapi, Kambing, Dan Skim) Pada Pengencer Tris Kuning Telur Terhadap Kualitas Spermatozoa Kerbau Murrah (Bubalus Bubalis)

Abstract

This study aimed to determine the effect of adding various types of milk (cow’s milk, goat’s milk, and skim milk) to tris egg yolk extender on the quality of Murrah buffalo (Bubalus bubalis) spermatozoa after the freezing process. The research was conducted at the Artificial Insemination Center (UPTD IB) of North Sumatra using a Completely Randomized Design (CRD) in a factorial pattern with two factors: type of milk (3 types) and concentration (3%, 6%, and 9%), each with 3 replications, resulting in 27 experimental units. Parameters observed included motility, viability, and abnormality of spermatozoa after thawing. The results showed that both the type of milk and its concentration had a highly significant effect (P<0.01) on all three parameters. The use of 9% cow’s milk yielded the best results, with motility at 34.2%, viability at 43.2%, and abnormality at 15.3%, which are still within the normal distribution limits for frozen semen. Conversely, the 9% skim milk treatment showed the lowest motility and viability values and the highest abnormality compared to other treatments. The nutritional content and fat composition of each milk type are believed to influence spermatozoa quality after freezing. Therefore, the combination of 9% cow’s milk in tris egg yolk extender is the most effective in maintaining the quality of frozen Murrah buffalo semen.