Isolasi dan Identifikasi Golongan Senyawa Flavonoida dari Kulit Batang Matoa (Pometia pinnata J.R. Forst & G. Forst)

Abstract

Traditionally,matoa plant was utilized as a medicinal remedy by local communities. The benefits of matoa are associated with the presence of secondary metabolites, namely flavonoids. The isolation and identification of flavonoid compounds from the stem bark of matoa (Pometia pinnata J.R. Forst & G. Forst) was carried out through extraction maceration. The powdered stem bark of matoa was extracted by maceration using methanol as the solvent. The methanol extract was fractionated with ethyl acetate, and the ethyl acetate extract was further fractionated with n-hexane to obtain the total flavonoids. The total flavonoid extract was then analyzed using Thin Layer Chromatography (TLC) and further separated by column chromatography with silica gel as the stationary phase and chloroform : ethyl acetate (90:10, 80:20, 70:30, 60:40, 50:50, 30:70, 20:80, 10:90) v/v as the mobile phase. The pure compound obtained is a yellow amorphous solid, has a mass of 5 mg and an Rf 0.66 with chloroform:ethyl acetate eluent 20:80 v/v. UV-Vis spectroscopic analysis with methanol solvent showed two absorptions with wavelengths (λmax) 318 nm and 270 nm. The FT-IR spectrum showed the presence of OH, C–H sp³ stretching, C=O ketone, C-C aromatic, C–H sp³ bending, C-O-C stretching asymmetric dan symmetric and C–H sp² out-of-plane at wavenumbers 3444.87; 2924.09; 1728.22; 1614.42; 1381.03; 1274.95; 1074.35; and 742.59 (cm⁻¹), respectively. The Proton Nuclear Magnetic Resonance (1H-NMR) spectrum showed the presence of the H-2 proton at a chemical shift of δ8.11 ppm as a singlet peak characteristic of isoflavone. Based on the interpretation of the spectroscopic data, the isolated compound were isoflavone group.