Mikropropagasi Tunas Kencur Hitam (Kaempferia parviflora) dalam Media MS dengan Penambahan BAP dan NAA

Abstract

Black galingale is a rhizome that can be used as a traditional medicinal ingredient and has begun to be cultivated in Indonesia. A major constraint in its cultivation is the provision of rhizomes as planting material. Propagation through shoot micropropagation can accelerate the supply of planting material. This study aimed to determine the effect of BAP and NAA supplementation as well as their interactions on the shoot micropropagation of black kencur. The research was conducted at the Alifa Agricultural Research Center (ALIFA-ARC) from March to June 2025. A Completely Randomized Design (CRD) with two treatment factors was used. The first factor was BAP concentration consisting of 0 ppm, 2 ppm, 4 ppm, and 6 ppm. The second factor was NAA concentration consisting of 0 ppm, 1 ppm, and 2 ppm. Observed parameters included the time of shoot emergence, number of shoots, shoot length, number of roots, and number of leaves. The best treatment combinations were obtained at 6 ppm BAP + 1 ppm NAA for average shoot emergence time, 6 ppm BAP + 0 ppm NAA for average number of shoots and leaves, 2 ppm BAP + 1 ppm NAA for average shoot length, and 4 ppm BAP + 0 ppm NAA for average number of roots.