Isolasi dan Sekuensing Fragmen cDNA Gen pi-d2 sebagai Ketahanan terhadap Penyakit Blas pada Padi Sigudang (Oryza sativa L.) dari Sumatera Utara

Abstract

Rice blast disease caused by the fungus Pyricularia oryzae is one of the major threats to rice production. Several resistant genes have been identified to confer resistance against blast disease, including the pi-d2 gene. The pi-d2 gene encodes a Receptor-Like Kinase (RLK) protein involved in disease resistance mechanisms. This study aimed to isolate and sequence a cDNA fragment of the pi-d2 gene associated with blast resistance in Sigudang rice (Oryza sativa L.) from North Sumatra. Total RNA was isolated from rice leaves then synthesized into cDNA for amplification. A pi-d2 gene fragment with a final length of 546 bp was successfully amplified and sequenced. BLASTn analysis showed that the fragment exhibited 100% identity when compared to the pi-d2 gene sequence of the rice cultivar Leung Pratew (KP738455.1). The results of phylogenetic analysis of pi-d2 gene fragments from Sigudang rice with 4 rice cultivars in GenBank (MZ570869.1, KP738455.1, KX791057.1, KP738452.1) have 0 genetic distance indicating a conserved region. The pi-d2 gene fragment of Sigudang rice has been registered in Genbank with the accession code PX219890.1.