Isolasi dan Sekuensing Fragmen cDNA Gen pi-ta sebagai Ketahanan terhadap Penyakit Blas pada Padi (Oryza sativa L.) Sigudang dari Sumatera Utara

Abstract

Blast disease caused by Pyricularia oryzae is one of the main threats to rice production in Indonesia. Several local rice varieties in North Sumatra, including the Sigudang cultivar, showed resistant to blast disease. The resistant is caused by the presence of resistance genes. One important of the gene is pi-ta, which plays a role in recognising the Avr-Pita gene in Pyricularia oryzae to produce NBS-LRR protein as a trigger for resistance. This study aimed to isolate and to sequence a cDNA fragment of the pi-ta gene in Sigudang rice. Total RNA was successfully isolated and used as a template for a cDNA synthesis. A cDNA fragments of the pi-ta gene were isolated and amplified, then sequenced. The edited sequencing results obtained a size of 639. The results of the analysis based on the sequence similarity of the pi-ta gene fragment of Sigudang rice with the cultivar Katy rice (GQ918480.1) using BLASTn showed an identity percentage of 100%. The results of phylogenetic analysis of the pi-ta gene fragment of Sigudang rice with 5 other rice varieties available in GenBank (GQ918487.1, GQ918447.1, GQ918406.1, GQ918452.1, GQ918480.1) have 0 genetic distance indicating a conserved region. The pi-ta gene fragment of Sigudang rice has been registered in GenBank with accession number PX442560.1.