Pengaruh Pemberian Ekstrak Kulit Buah Manggis (Garcinia Mangostana L) terhadap Marker Inflamasi, Eritropoietin dan Gambaran Histopatologi Ginjal Tikus Wistar Model Penyakit Ginjal Kronik

Abstract

Chronic Kidney Disease (CKD) is a global health problem whose prevalence continues to increase, with a global prevalence of 10%. Most CKD patients exhibit a chronic inflammatory state with elevated levels of inflammatory markers, such as Interleukin-6 (IL-6), as well as increased prevalence associated with decreased kidney function. Serum hepsidin was significantly higher in haemodialysis (HD) patients compared to controls. In HD patients, inflammation has been associated with Erythropoietin (EPO) resistance, mainly due to inflammatory conditions. Plasma markers of oxidative stress were elevated in CKD patients, indicating an increase in systemic oxidative stress, as measured by Malondialdehyde (MDA) examination. The peel of the mangosteen fruit (Garcinia mangostana L) has been used to treat various diseases, including skin diseases, bacterial infections, and inflammation, as well as to reduce IL-6 levels and lower MDA. This study aims to analyze the effect of giving mangosteen peel extract (Garcinia mangostana L) on inflammatory markers (levels of Interleukin-6 (IL-6), Hepcidin, MDA) and Erythropoietin, as well as the histopathological picture of kidney disease in Wistar rats modeled chronic kidney disease. Methods: Male rats (Rattus norvegicus) aged 12 weeks were divided into 6 groups: normal, negative control (Adenine 600 mg/kgBB 10 days p.o), positive control (Adenine 600 mg/kgBB 10 days p.o + vit. C 45 mg/kgBB 1 time a day, 28 days.p.o), P1 treatment group (Mangosteen Peel Extract (MPE) 200 mg/kgBB, 28 days, p.o), P2 (MPE 400 mg/kgBB, 28 days, p.o), and P3 (MPE 600 mg/kgBB, 28 days, p.o). The design used is the Posttest Only Control Group Design Results: There was no effect of mangosteen peel extract on IL-6, Hepcidin, EPO, and MDA levels, as well as inflammatory scores from the histopathology of the kidneys of Wistar rats (p > 0.05). There was a significant influence on the progression of kidney function, in the form of a decrease in Urea levels between Negative Control and the P1 and P2 Groups (39.6±2.93 vs 29 ± 1.14 vs 28.2 ± 1.07, p=0.012, respectively) and Creatinine levels between Negative Control and P1, P2 and P3 Groups (0.83 ± 0.14 vs 0.46 ±0.4, vs 0.45 ± 0.05, vs 0.5 ± 0.09, p=0.047, respectively). Conclusion: No effect of mangosteen peel extract on inflammatory markers, erythropoietin, and renal histopathology of Wistar rats, Chronic Kidney Disease model