| dc.description.abstract | Periodontal disease is an inflammatory condition of the tooth-supporting tissues
caused by the accumulation of plaque and biofilm. One of the primary bacteria involved
in the progression of periodontitis is Porphyromonas gingivalis, which is capable of
forming subgingival biofilm. Use of mouthwash as an adjunctive therapy is essential.
However, chemical mouthwashses such as chlorhexidine are associated with several
side effects, creating a need for safer herbal alternatives. One promising option is
pomegranate peel extract (Punica granatum L.), which is known to possess
antibacterial and antibiofilm activities. This study aimed to evaluate the effectiveness
of pomegranate peel extract mouthwash in inhibiting P. gingivalis biofilm formation
in vitro and to determine the most effective concentration. This laboratory experimental
study employed a post-test only control group design. The sample consisted of P.
gingivalis ATCC®33277. The mouthwash formulations contained pomegranate peel
extract at concentrations of 6,25%, 12,5%, and 25%, with 0,2% chlorhexidine
gluconate as the positive control and aquadest as the negative control. Antibiofilm
testing was conducted using a 96-well microtiter plate method with crystal violet
staining, and biofilm density was measured by Optical Density at 490 nm. The results
showed significant differences among the groups (p<0,05). The percentage of biofilm
inhibition increased with extract concentration: 44,44% at 6,25% concentration,
46,30% at 12,5% concentration, and 76,67% at 25% concentration. The pomegranate
peel extract mouthwash is effective in inhibiting the formation of P. gingivalis
biofilm in vitro, with the 25% concentration being the most potential formulation to
be developed as an alternative herbal mouthwash. | en_US |
