Uji Potensi Gel Yang Mengandung Kitosan Karboksimetil, Hyaluronic Acid, Dan Kolagen Terhadap Pertumbuhan Bakteri Porphyromonas Gingivalis Dan Uji Viabilitas Sel Fibroblas (In Vitro)

Abstract

Periodontitis is closely associated with the bacterium Porphyromonas gingivalis, making the development of biocompatible antimicrobial agents important for periodontal therapy. This study evaluated the antibacterial effect of a gel containing carboxymethyl chitosan, hyaluronic acid, and collagen, as well as its cytotoxicity on 3T3 fibroblast cells. Antibacterial activity was assessed using the Kirby–Bauer method on Brucella Agar with 5% sheep blood, while cytotoxicity was evaluated with the MTT assay according to ISO 10993-5. The gel produced an inhibition zone of 16.90 mm, indicating strong antibacterial activity. Fibroblast cell viability reached 90,32%, classifying the material as non-cytotoxic. In conclusion, the combination gel demonstrates strong antibacterial effects against P. gingivalis and exhibits good biocompatibility, supporting its potential as a local therapeutic agent in periodontal treatment.