Efektivitas Penggunaan Film Kitosan Cangkang Kepiting Hitam (Scylla serrata) Terhadap Penyembuhan Luka Bakar Pada Mukosa Rongga Mulut Tikus Galur Wistar (Rattus norvegicus)

Abstract

A wound is a condition where the epithelial layer of the skin or mucosa is disrupted due to physical or thermal damage, such as burns wound. In the oral mucosa, it can be caused by contact with substances at high temperatures (heat) and very low temperatures (cold). Although these wounds can heal on their own, they take a relatively long time, are uncomfortable, and can lead to other complications. Giant mud crab shells are a natural ingredient that contains the highest amount of chitin, which can be processed into chitosan, thus having potential in wound healing. This study aims to assess the effectiveness of giant mud crab chitosan film in promoting the healing of oral mucosal burns in Wistar rats. This type of research is s truly experimental using 30 male Wistar rats as samples which were divided into 5 groups, namely the positive control group using aloclair gel, 1% chitosan film, 2% chitosan film, 3% chitosan film, and negative control using CMC-Na placebo gel. The study was conducted by observing erythema, edema, and wound closure on days 1, 3 and 7. Based on the results of the Kruskal-Wallis and Mann-Whitney test, the use of 1%, 2%, and 3% chitosan films was effective in healing burns in the oral mucosa of Wistar rats.