Pengaruh Penambahan Etil Glikol dan Gliserol terhadap Kualitas DNA Sperma Ikan Batak (Tor soro) pada Pasca Kriopreservasi

Abstract

Batak fish (Tor soro) is an endemic fish of Danau Toba whose population continues to decline due to overexploitation, habitat destruction, and introduced predators. Conservation efforts can be carried out with sperm cryopreservation technology to provide quality sperm throughout the year. This study aims to analyze the type of appropriate cryoprotectant solution, and determine the best concentration, as well as evaluate the effect of Ethyl Glycol and glycerol on the quality of Batak fish sperm DNA. The study used two types of cryoprotectants (ethyl glycol and Glycerol) with 7 different concentrations (0%; 2.5%; 5%; 7.5%; 10%; 12.5%; and 15%). The parameters observed included motility, viability, and DNA integrity, with ANOVA analysis and Duncan's test. The results showed that the type of solution, concentration, and their interaction had a significant effect (P <0.05) on the motility and viability of Batak fish sperm. Glycerol concentration of 7.5% produced the highest value with 28.67% motility and 63.00% viability, while Ethyl Glycol was best at 0% concentration with 23.33% motility and 59% viability. DNA electrophoresis results showed clear and intact DNA bands without fragmentation, indicating that these two cryoprotectants did not damage genetic integrity. Thus, Glycerol 7.5% is the most effective concentration for cryopreservation of junung fish sperm. Meanwhile, 0% ethyl glycol also gave good results without damaging DNA.