Efektivitas Antiinflamasi Ekstrak Daun Petai Cina (Leucaena leucocephala) terhadap Kadar Interleukin-6 (IL-6) pada Tikus Wistar Model Ulkus Traumatikus

Abstract

Introduction. Traumatic ulcers are common lesions in Indonesia, arising from inflammation in the oral cavity. Petai Cina (Leucaena leucocephala), a tropical plant native to Indonesia, contains flavonoids that act as anti-inflammatory agents by reducing levels of pro-inflammatory cytokines, including interleukin-6 (IL-6). Purpose of this study was to determine the effectiveness of petai cina leaf extract with concentrations of 15%, 30%, and 45% in reducing clinical inflammatory symptoms in thermal traumatic ulcers, to analyze the effect of petai cina leaf extract on interleukin-6 (IL-6) levels, and to analyze the relationship between IL-6 levels and clinical symptoms of inflammation. Method This study is a purely experimental in vivo study, with a post-test only control group design. This study used 30 male Wistar rats divided into groups with traumatic ulcers without treatment, povidone iodine treatment, and treatment with 15%, 30%, and 45% Chinese petai leaf extract gel for 7 days. IL-6 levels were examined using the ELISA technique from Wistar rat traumatic ulcer tissue samples. The results of the study based on descriptive tests showed significant differences in the mean values ​​of clinical symptoms of ulcer diameter length, rubor, tumor, and dolor, as well as different IL-6 levels in each treatment group (p<0.05). The Kruskal-Wallis test showed a significant relationship between clinical symptoms of ulcers and IL-6 levels with opposite gel concentrations, as well as a significant relationship between clinical symptoms of ulcers and IL-6 levels that were unidirectional. The conclusion of this study is that there is an effect of petai cina leaf extract gel concentrations of 15%, 30%, and 45% on clinical symptoms of inflammation and IL-6 levels in Wistar rats, a model of traumatic ulcers. There was a significant, one-way relationship between IL-6 levels and clinical symptoms of traumatic ulcers. The results of the treatment group that most closely matched the positive control group with povidone iodine were those with 45% petai cina leaf extract gel.