Potensi Khamir Endofit Daging Buah Nanas Madu (Ananas Comosus) Sebagai Penghasil Asam Indol Asetat (IAA) dan Pelarut Fosfat

Abstract

This study aims to obtain endophytic yeasts isolated from pineapple (Ananas comosus) fruit flesh and to evaluate their potential for indole acetic acid (IAA) production and phosphate solubilization. The yeasts were isolated using a dilution method on malt extract agar medium and characterized morphologically. The production of IAA by each yeast isolate was observed qualitatively using Salkowski reagent and the potential of the hormone as a plant growth was examined on root of Coleus sp. Phosphate solubilization was determined based on the formation of a clear zone surrounding yeast colonies on Pikovskaya’s medium. The result found six yeast isolates were observed with varying colony characteristics, both morphologically and microscopically. The yeast with the highest IAA production was found on isolate YEAC-03 with a concentration of 0.627 mg/L. Indole acetic acid produced by all yeast isolates have significant effect root length and no effect on roots number. Yeast isolate YEAC-06, produce IAA 0.320 mg/L capable of producing longer roots than the others. Yeast isolate YEAC-06 (3.05) was the highest phosphate solubilization index followed by isolate YEAC-05 (3.00). The ability of endophytic yeast to produce IAA and to dissolve phosphate indicate their potential to be used as a root stimulant and biofertilizer for plants growth.