Pengaruh Berbagai Konsentrasi BAP dan NAA terhadap Multiplikasi Tunas Pisang Barangan Merah (Musa acuminate colla) Secara In Vitro.

Abstract

The Barangan banana (Musa acuminate Colla) originates from North Sumatra and is distributed throughout Indonesia. The main problem in banana cultivation is the availability of seeds. Banana plants are generally propagated by suckers, making it difficult to obtain large numbers of seeds in a relatively short time. The resulting seeds are not uniform and are not free from pests and diseases. In vitro plant culture is a rapid method for plant propagation in an aseptic environment. This method can produce high-quality plants with characteristics consistent with the parent plant and free from pests and diseases. This study aims to determine the effect of various concentrations of BAP and NAA in MS medium on shoot multiplication of red Barangan banana (Musa acuminate colla). This research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, University of North Sumatra, from June to October 2025. A completely randomized design (CRD) was used with two factors. The first factor was the concentration of benzyl aminopurine (BAP): B0 (0 ppm), B1 (4 ppm), and B2 (6 ppm). The second factor was the concentration of naphthalene acetic acid (NAA): N0 (0 ppm), N1 (1 ppm), and N2 (2 ppm). Parameters observed in this study included the percentage of live explants (%), age at shoot emergence, number of shoots, shoot length, number of roots, root length, and number of leaves. The results showed that the BAP concentration significantly affected the age at shoot emergence, number of shoots, and number of leaves. The interaction between BAP and NAA significantly affected shoot length, number of roots, and root length.