Karakterisasi Enzim Karbofuran Hidrolase dari Bacillus Cereus Strain HS06 dan Bacillus Paramycoides Strain BSAS15

Abstract

Bacillus cereus strain HS06 and Bacillus paramycoides strain BSAS15 are two bacteria known to have the ability to degrade carbofuran, an active compound used in pesticides which are very dangerous to the environment. This study aims to determine the characteristic of the carbofuran hydrolase extracelllular and intracellular from B. cereus strain HS06 and B. paramycoides strain BSAS15. Extracelllular and intracellular enzyme of carbofuran hydrolase were isolated from B. cereus strain HS06 and B. paramycoides strain BSAS15 cultures, enzyme activity was measured and tested for the effect of temperature (30-50oC), pH (5-9) and metals (Na, Ca, Fe, Mg, Mn). One unit of carbofuran hydrolase enzyme activity is defined as the amount of enzyme that can produce 1 mol of carbofuran-7-phenol per minute per ml of enzyme with carbofuran as the substrate. The results indicate that the carbofuran hydrolase intracellular enzyme produced by B. cereus and B. paramycoides has a higher activity than that from extracellular enzymes. The intracellular enzyme activiy of B. cereus has an optimum condition at 40°C, pH 9, and an increase in enzyme activity with the addition of Fe metal ions. Meanwhile, B. paramycoides at 40°C, pH 7, and with the addition of Fe metal. The carbofuran hydrolase enzyme from B. cereus and B. paramycoides have different optimal effectiveness characteristics with higher activity in its intracellular than the extracellular enzymes.