Analisa Kandungan Protein dan Amilosa pada Galur Kedelai [Glycine Max (L.) Merr.] Hasil Persilangan

Abstract

This study aimed to analyze the protein and amylose content of eight F6 soybean (Glycine max L.) lines resulting from crosses of the first six (F6) generation. The study was conducted in the Plant Biotechnology Laboratory of the Faculty of Agriculture, Universitas Sumatera Utara, Medan, at an altitude of approximately 25 meters above sea level in July. A non-factorial completely randomized design (RBD) was used, using F6 soybean lines derived from crosses of three parents and a mutation line. The Bradford method was used for protein analysis, while amylose was analyzed spectrophotometrically. The results showed that the DEVON 2 line had the highest protein content (42.86%), and DERAP 1 the lowest (15.49%). The highest amylose content was found in DEGA 1 (23.83%), and the lowest in ANJ 2 (12.66%). The differences between the lines indicate significant genetic diversity. The DEVON 2 and DEGA 1 lines have the potential to be developed to meet the needs of the food industry.