Hubungan Jumlah Sel Mast Dengan Tampilan Ekspresi Vascular Endothelial Growth Factor (VEGF) pada Cutaneous Squamous Cell Carcinoma

Abstract

Latar Belakang: Kanker kulit non melanositik tersering adalah basalioma diikuti squamous cell carcinoma. Squamous cell carcinoma merupakan keganasan epitel skuamus. Sel mast merangsang neovaskularisasi dan angiogenesis pada beberapa tumor. Salah satu faktor utama angiogenesis pada squamous cell carcinoma adalah Vascular Endothelial Growth Factor (VEGF). Metode: 30 blok parafin dengan diagnosis cutaeous squamous cell carcinoma di warnai dengan CD117 dan VEGF. Sel mast dihitung dari akumulasi terbanyak satu lapangan pandang pada pembesaran 200x, diberi skor <15 sel atau ≥ 15 sel. Ekspresi VEGF berdasarkan penjumlahan intensitas pewarnaan dan persentase sel yang terpulas positif. Hasil: Jumlah Sel mast ≥ 15 sel sebanyak 7 kasus (23,3%). Ekspresi VEGF terekspresi positif pada 30 kasus (100%). Uji kesesuian Fischer’s Exact Test memiliki nilai yang tidak signifikan (p>0,05) Kesimpulan: Tidak terdapat kesesuaian hubungan jumlah sel mast dengan ekspresi VEGF dari p-value, namun kemungkinan terdapat hubungan belum dapat disingkirkan.

squamous cell carcinoma. Squamous cell carcinoma is a malignancy of squamous epithelium. Mast cells stimulate neovascularization and angiogenesis in multiple tumors. One of the main factors of angiogenesis in squamous cell carcinoma is the Vascular Endothelial Growth Factor (VEGF). Method: Formalin-fixed paraffin-embedded sections from 30 cutaneous squamous cell carcinoma were obtained. Mast cells are calculated from the accumulated most of the field of view at 200x magnification, by a score of <15 cells or ≥ 15 cells. VEGF expression is based on the sum of staining intensity and percentage of positive cells smeared. Results: The number of mast cells ≥ 15 cells were 7 cases (23.3%). Expressed VEGF positive expression in 30 cases (100%). Suitability test Fischer's Exact Test has value not significant (p> 0.05). Conclusions: There is no conformance relationship number of mast cells with VEGF expression of p-value, but it is likely there is a relationship can not be ruled out.