Hubungan Tumor-Infiltrating Lymphocytes (TILs) dengan Lokasi Massa Tumor dan Grading Histologi Karsinoma Sel Skuamosa Kulit

Abstract

Background. Cutaneous squamous cell carcinoma is the malignant tumor derived from the keratinocytes of the epidermis. It is the second most common skin cancer in Indonesia. Tumor-infiltrating lymphocytes (TILs) have been studied and related with the prognostic factor for many solid tumors. The research of TILs with Hematoxylin&Eosin staining for cutaneous squamous cell carcinoma has not been studied until nowadays. Objective. To analyze the correlation between TILs and the tumor mass location and histological grading of cutaneous squamous cell carcinoma. Material and Methods. This is an analytical research with cross sectional approach using the slide and the paraffine block from the patients diagnosed as cutanoeus squamous cell carcinoma stained with Hematoxylin&Eosin. The statistical analysis with Kruskal-Wallis method is used to analyze the correlation of intratumoral and stromal TILs with the tumor mass location and histological grading of cutaneous squamous cell carcinoma. Results. Based on the statistical analysis with Kruskal-Wallis method for 48 samples of the patients with cutaneous squamous cell carcinoma to analyze the correlation of intratumoral TILs and stromal TILs with the tumor mass location, the p-value is 0,824 and 0,124 (P>0,05). The correlation of intratumoral and stromal TILs with the histological grading show the p-value of 0,058 (p>0,05) and 0,027 (p<0,05) Conclusion. Only the stromal TILs is closely related to the histological grading of cutaneous squamous cell carcinoma.

Latar Belakang. Karsinoma sel skuamosa kulit merupakan tumor ganas yang berasal dari keratinosit epidermis dan merupakan keganasan kulit kedua terbanyak di Indonesia. Tumor-infiltrating lymphocytes (TILs) telah banyak diteliti dan berhubungan dengan faktor prognostik untuk beberapa tumor padat. Penelitian TILs dengan pewarnaan Hematoxylin&Eosin untuk karsinoma sel skuamosa kulit belum pernah dilakukan sampai saat ini. Tujuan. Menganalisis hubungan tingkat TILs dengan lokasi massa tumor dan grading histologi karsinoma sel skuamosa kulit. Bahan dan Metode. Penelitian analitik dengan pendekatan cross-sectional menggunakan slide dan blok parafin penderita karsinoma sel skuamosa kulit dengan pewarnaan Hematoxyline&Eosin. Analisis statistik dilakukan dengan uji Kruskal-Wallis untuk melihat hubungan intratumoral dan stromal TILs dengan lokasi massa tumor dan grading histologi karsinoma sel skuamosa kulit. Hasil. Berdasarkan analisis statistik Kruskal-Wallis terhadap 48 sampel karsinoma sel skuamosa kulit dengan menguji hubungan intratumoral TILs dan stromal TILs terhadap lokasi massa tumor, diperoleh nilai p-value sebesar 0,824 dan 0,124 (p > 0,05) sedangkan hubungan intratumoral TILs dan stromal TILs terhadap grading histologi menunjukkan nilai p-value masing-masing sebesar 0,058 (p > 0,05) dan 0,027 (p < 0,05). Simpulan. Hanya stromal TILs yang menunjukkan hubungan bermakna dengan grading histologi karsinoma sel skuamosa kulit.