Uji Daya Hambat Asap Cair (Liquid Smoke) Hasil Pirolisis Residu Kopi Arabika Terhadap Bakteri Staphylococcus Aureus pada Abses Odontogenik
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2020Author
Wahab, Michelle Natascha
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Odontogenic abscess is a pathological cavity that contains a thick fluid called pus which could increase the rate of morbility and mortality. Staphylococcus aureus, the main bacteria that causes odontogenic abscess has been resistant to various types of antibiotics. One of the alternative treatments is using liquid smoke which is the result of incomplete combustion condensation (pyrolysis) from natural organic material which has the main components, such as hemicellulose, cellulose, and lignin, so that, it established antibacterial substances in the form of phenol compounds, carbonyl, and organic acids. Arabica coffee residue contain the main components above, so that it is suitable to be used as raw material for producing liquid smoke to inhibit the growth of Staphylococcus aureus in odontogenic abscess.
The design of this research was true experimental randomized posttest only control group design to know the inhibitory power and optimal concentration of liquid smoke from arabica coffee residue pyrolysates against Staphylococcus aureus. It was initiated by manufacturing liquid smoke through the pyrolysis of arabica coffee residue. The result of pH measurement of liquid smoke was 2,8. Inhibition zone diameter of liquid smoke 6.25%, 12.5%, 25%, 50%, dan 100% concentrated was 16.44 mm, 16.22 mm, 17.74 mm, 21.47 mm, dan 21.20 mm. Data analysis showed that liquid smoke from arabica coffee residue pyrolysates could inhibit Staphylococcus aureus growth with an optimal concentration 50%. Abses odontogenik merupakan suatu rongga patologis yang berisi nanah (pus) yang dapat meningkatkan angka morbilitas dan mortalitas. Bakteri Staphylococcus aureus adalah penyebab utama abses odontogenik yang telah resisten terhadap berbagai jenis antibiotik. Salah satu perawatan alternatifnya adalah dengan aplikasi asap cair (liquid smoke) yang merupakan hasil kondensasi pembakaran tidak sempurna (pirolisa) dari bahan organik alamiah yang memiliki komponen utama, yaitu hemiselulosa, selulosa, dan lignin sehingga membentuk zat antibakteri berupa senyawa fenol, karbonil, dan asam-asam organik. Residu kopi arabika mengandung komponen utama pembentuk asap cair yang bersifat antibakteri, sehingga sangat cocok dimanfaatkan sebagai bahan baku penghasil asap cair untuk menghambat pertumbuhan bakteri Staphylococcus aureus pada abses odontogenik.
Penelitian ini berjenis eksperimental dengan rancangan penelitian true experimental randomized posttest only control group design untuk mengetahui daya hambat asap cair hasil pirolisis residu kopi arabika terhadap bakteri Staphylococcus aureus. Pelaksanaannya dimulai dengan pembuatan asap cair hasil pirolisis residu kopi arabika. Asap cair yang diperoleh ber-pH 2,8. Diameter uji daya hambat asap cair berkonsentrasi 6,25%, 12,5%, 25%, 50%, dan 100% terhadap bakteri Staphylococcus aureus adalah 16,44 mm, 16,22 mm, 17,74 mm, 21,47 mm, dan 21,20 mm. Analisis data menunjukkan bahwa asap cair hasil pirolisis residu kopi arabika mampu menghambat pertumbuhan bakteri Staphylococcus aureus, dimana konsentrasi optimalnya adalah 50%.
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