Perbedaan Heat Shock Protein 60 Pada Pasien Gastritis H.Pylori Positif Dan Negatif Serta Korelasinya Dengan Neutrofil Dan Limfosit Rasio

Abstract

Background: Gastritis is a disease characterized by an inflammatory process in both the mucosal and submucosal lining of the stomach. Helicobacter pylori (H.pylori) binds to gastric and mucin epithelial cells via HSP60, and the adaptive immune system against HSP60 is induced in patients infected with H. Pylori. Objective: To determine the comparison of serum HSP60 levels between positive and negative H. pylori gastritis patients and their correlation with neutrophillymphocyte ratio. Research Method: Cross-sectional design. Sampling was carried out at the Endoscopy Unit of Adam Malik General Hospital Medan. The study sample was taken as many as 60 subjects consecutively. The independent variable of the study was H. pylori infection and the dependent variable was HSP60 and neutrophillymphocyte ratio. Data analysis used the Mann Whitney U test and the Spearman correlation test. Results: The demographic characteristics of the study subjects were 36 male subjects (60%), the median value of age 47.7 (25-66) years, the highest educational history was 34 subject high schools (56,7%), the most work history was private employees 24 subject (40%). There was a difference in HSP60 in patients with positive and negative H.pylori gastritis (p <0,000). There was no correlation between HSP60 and neutrophil-lymphocyte ratio in patients with positive and negative H. pylori gastritis (p <0.121). Conclusion: There are differences in HSP60 in patients with positive and negative H.pylori gastritis

Latar belakang: Gastritis merupakan penyakit yang ditandai dengan proses inflamasi baik pada lapisan mukosa maupun submukosa lambung. Helicobacter pylori(H.pylori) berikatan dengan sel epitel lambung dan musin melalui HSP60, dan sistem imun adaptif terhadap HSP60 diinduksi pada pasien terinfeksi H. Pylori. Tujuan: Untuk mengetahui perbandingan kadar serum HSP60 antara pasien gastritis H. pylori positif dan negatif serta korelasinya dengan netrofil- limfosit rasio. Metode Penelitian: Desain potong lintang. Pengambilan sampel dilakukan di Unit Endoskopi RSUP H. Adam Malik Medan. Sampel penelitian diambil sebanyak 60 subjek secara konsekutif. Variabel independen penelitian adalah Infeksi H. Pylori dan variabel dependen adalah HSP60 dan netrofil- limfosit rasio. Analisis data menggunakan uji U Mann Whitney dan uji korelasi Spearman. Hasil Penelitian: karakteristik demografi subjek penelitian adalah laki-laki 36 subjek (60%), nilai median usia 47.7(25-66) tahun, riwayat pendidikan terbanyak yaitu SMA sebanyak 34 subjek (56,7%), riwayat pekerjaan terbanyak yaitu pegawai swasta 24 subjek (40%). Terdapatnya perbedaan HSP60 pada pasien gastritis H.pylori positif dan negatif (p < 0,001). Tidak terdapat korelasi antara HSP60 dengan neutrofil-limfosit rasio pada pasien gastritis H.pylori positif dan negatif (p < 0,121). Kesimpulan: Terdapatnya perbedaan HSP60 pada pasien gastritis H.pylori positif dan negatif