Analisis Imunostimulan Ekstrak Etanol Daun Pirdot (Saurauia vulcani Korth.) pada Tikus (Rattus norvegicus L.)

Abstract

The purpose of this study was to analyze ethanol extract of pirdot leaves in rats. The parameters evaluated were rat erythrocytes, leukocytes, lymphocytes, IgG, Interleukin-2, activity, spleen histopathology, liver histopathology, and kidney Phistopathology. As an antigen, sheep red blood cells are given. In this study twenty-four rats aged three months were used with a weight of 150 to 200 grams. Rats divided into four treatments each consisted of six. The first treatment was given CMC (Control), the cecond treatment was given Lamb Red Blood Cells, the third treatment was given Saurauia ethanol extract 500 mg/bb, the fourth treatment was given EES 500 mg/bb+SRBC (sheep red blood cells). The provision of SRBC was carried out on the 8th and 15th days. Saurauia ethanol extract (SEE) treatment is given for 30 days. On the 31st day blood was taken by decapitation to analyze the erythrocytes, leukocytes, lymphocytes and serum. For analysis of IgG and IL-2 obtained from serum. Histopathology of organs (spleen, liver and kidney) made preparations with staining of hematoxylin-eosin. IgG and IL-2 data were used with the Elisa technique. The results showed that erythrocytes and lymphocytes increased from control while leukocytes decreased from control. IgG increased significantly in the treatment of Saurauia ethanol extract and were significant with SEE + SRBC treatment. IL-2 decreased from control SaurauiaThe purpose of this study was to analyze ethanol extract of pirdot leaves in rats. The parameters evaluated were rat erythrocytes, leukocytes, lymphocytes, IgG, Interleukin-2, activity, spleen histopathology, liver histopathology, and kidney Phistopathology. As an antigen, sheep red blood cells are given. In this study twenty-four rats aged three months were used with a weight of 150 to 200 grams. Rats divided into four treatments each consisted of six. The first treatment was given CMC (Control), the cecond treatment was given Lamb Red Blood Cells, the third treatment was given Saurauia ethanol extract 500 mg/bb, the fourth treatment was given EES 500 mg/bb+SRBC (sheep red blood cells). The provision of SRBC was carried out on the 8th and 15th days. Saurauia ethanol extract (SEE) treatment is given for 30 days. On the 31st day blood was taken by decapitation to analyze the erythrocytes, leukocytes, lymphocytes and serum. For analysis of IgG and IL-2 obtained from serum. Histopathology of organs (spleen, liver and kidney) made preparations with staining of hematoxylin-eosin. IgG and IL-2 data were used with the Elisa technique. The results showed that erythrocytes and lymphocytes increased from control while leukocytes decreased from control. IgG increased significantly in the treatment of Saurauia ethanol extract and were significant with SEE + SRBC treatment. IL-2 decreased from control Saurauia ethanol extract can function as immunostimulant because it contains flavonoids and saponins. ethanol extract can function as immunostimulant because it contains flavonoids and saponins.

The purpose of this study was to analyze ethanol extract of pirdot leaves in rats. The parameters evaluated were rat erythrocytes, leukocytes, lymphocytes, IgG, Interleukin-2, activity, spleen histopathology, liver histopathology, and kidney Phistopathology. As an antigen, sheep red blood cells are given. In this study twenty-four rats aged three months were used with a weight of 150 to 200 grams. Rats divided into four treatments each consisted of six. The first treatment was given CMC (Control), the cecond treatment was given Lamb Red Blood Cells, the third treatment was given Saurauia ethanol extract 500 mg/bb, the fourth treatment was given EES 500 mg/bb+SRBC (sheep red blood cells). The provision of SRBC was carried out on the 8th and 15th days. Saurauia ethanol extract (SEE) treatment is given for 30 days. On the 31st day blood was taken by decapitation to analyze the erythrocytes, leukocytes, lymphocytes and serum. For analysis of IgG and IL-2 obtained from serum. Histopathology of organs (spleen, liver and kidney) made preparations with staining of hematoxylin-eosin. IgG and IL-2 data were used with the Elisa technique. The results showed that erythrocytes and lymphocytes increased from control while leukocytes decreased from control. IgG increased significantly in the treatment of Saurauia ethanol extract and were significant with SEE + SRBC treatment. IL-2 decreased from control Saurauia ethanol extract can function as immunostimulant because it contains flavonoids and saponins.