Ekspresi L - Selectin pada Jaringan Endometriosis

Abstract

Objective : To determine the differences of L-Selectin Expression in ectopic endometrium from endometriosis patients compared with normal peritoneum. Method : This analytical cross sectional study recruited 30 paraffins blocks of ectopic endometrium tissue obtained from patients who undergo laparotomy or laparoscopy while 30 control paraffins blocks recruited from patients undergo tubal sterilization. Paraffin tissue contained in the managed and performed immunohistochemical staining using the lymp node as a positive control. The result were interpreted based on the intensity of the color and then statistically analyzed. Results : 30 cases of endometriosis were observed and there were 12 (40%) ectopic endometrium of endometriosis patients stained with +3 and + 2 in intensity while in 30 cases of normal peritoneum stained with negatif intensity. L Selectin expression in ectopic endometrium is higher than normal peritoneum and this difference was statistically significant (p<0.05). In the relation to L-Selectin expression and the degree of endometriosis, 4 (80%) of stage I endometriosis stained with +1 in intensity, 7 (58%) of stage II endometriosis stained with + 2 in intensity and 9 (69.2%) of stage 3 endometriosis stained with + 3 in intensity. L Selectin expression due to the stage of the endometriosis was found to be higher for each stage and this difference was statistically significant (p<0.05). Conclusion : The L-Selectin expression significantly differed between ectopic endometrium of endometriosis patients compared with non endometriosis and the L-Selectin expression was found to be higher for each stage of endometriosi .

Tujuan penelitian : Mengetahui perbedaan ekspresi L-Selectin pada jaringan endometrium ektopik penderita endometriosis dan jaringan peritoneum normal. Metode Penelitian : Penelitian ini merupakan penelitian analitik dengan rancangan Cross Sectional terhadap 30 parafin blok jaringan endometrium ektopik penderita endometriosis yang diperoleh dari tindakan laparotomi atau laparoskopi dan 30 parafin blok jaringan peritoneum normal yang diperoleh dari tindakan sterilisasi. Dilakukan pewarnaan imunohistokimia terhadap jaringan tersebut dengan menggunakan kelenjar getah bening sebagai kontrol positif. Hasil penelitian diinterpretasikan berdasarkan kekuatan intensitas warna dan dianalisa secara statistik. Hasil Penelitian : Dari 30 kasus endometriosis yang diamati, sebanyak 12 (40%) jaringan endometrium ektopik penderita endometriosis terwarnai dengan intensitas +3 dan +2 sedangkan 30 kasus dari peritoneum normal, keseluruhannya terwarnai dengan intensitas negatif. Ekspresi L-Selecin pada jaringan endometrium ektopik penderita endometriosis lebih tinggi dibandingkan peritoneum normal dan bermakna secara statistik (p<0.05). Berdasarkan perbandingan antara ekspresi l-Selectin dengan stadium endometriosis, sebanyak 4 (80%) endometriosis stadium 1 terwarnai dengan intensitas +1, 7 (58.3%) endometriosis stadium 2 terwarnai dengan intensitas +2 dan endometriosis stadium 3 terwarnai dengn intensitas +3, dengan (p<0.05) sehingga didapat adanya hubungan yang bermakna antara ekspresi L-Selectin dan Stadium endometriosis. Kesimpulan : Terdapat perbedaan yang bermakna antara ekspresi L-selectin pada kelompok Endometriosis dengan kelompok non Endometriosis dan terdapat perbedaan yang bermakna antara ekspresi L-Selectin dengan stadium Endometriosis