Hubungan Ekspresi Pulasan Imunohistokimia Protein Gene Product (PGP9.5) dengan Derajat Histopatologi Kanker Ovarium Tipe Adenokarsinoma Serosum dan Tipe Adenokarsinoma Musinosum

Abstract

Background : Ovarian cancer is a gynecological cancer found almost 30% of all cancers of the reproductive organs. The most common type of ovarian cancer that is derived from the ovarian surface epithelium. Differentiation into cell types of epithelial ovarian cancer derived from epithelial located on the same control that will determine epithelium in the female reproductive system organs during embryonic development. Protein Gene Product (PGP9.5) is a peptide specific to neurons which removes ubiquitin from ubiquinated proteins and can act as a promoter hipermetilasi that important in gene transcription in cancer Aim : To see PGP9.5 immunohistochemistry at various histopathological degrees of ovarian cancer serous adenocarcinoma types and mucinous adenocarcinoma types. Methods: This study is a descriptive analytic with cross sectional study approach. This study was conducted by taking and collecting 45 sample ovarian tissue paraffin blocks which have been diagnosed as ovarian cancer serous adenocarcinoma types and mucinous adenocarcinoma types, were stain with immunohistochemistry PGP9.5 and assessing the intensity of the expression in tumor cell. Result: Base on Kruskal-Wallis statistical analysis in 26 samples with negative immunohistochemistry PGP9.5 found 13 cases (50%) well diffrentiated, 6 cases (23,1%) moderately differentiated and 7 cases (21,9%) poorly differentiated. On 5 samples with low expression of PGP9.5, 4 cases (80%) well differentiated and 1 case (20%) poorly differentiated. On 4 cases with moderately expression, found 2 cases (50%) well differentiated, 1 case (25%) moderately differentiated and 1 case (25%) poorly differentiated. On 10 cases with strong expression of PGP9.5, found 2 cases (20%) well differentiated and 8 cases (80%) poorly differentiated. Conclusion: No relation between PGP9.5 expression with hystopatologycal grading of ovarian cancer serous adenocarcinoma types and mucinous types with p-value > 0.05.

Latar belakang : Kanker ovarium merupakan kanker ginekologi yang dijumpai hampir 30% dari semua kanker organ reproduksi. Tipe paling umum dijumpai adalah kanker ovarium yang berasal dari epitel permukaan ovarium. Differensiasi menjadi jenis sel-sel epitel pada kanker ovarium jenis epitel berada pada kontrol yang sama dan nantinya akan menentukan jenis epitel pada organ sistem reproduksi wanita selama perkembangan embrionik. Protein Gene Product (PGP9.5) merupakan suatu peptide spesifik pada neuron yang menghilangkan ubiquitin dari protein-protein ubiquinated dan dapat berperan sebagai promoter hipermetilasi yang penting dalam gen transkripsi pada kanker. Tujuan : Melihat hubungan ekspresi imunohistokimia PGP9.5 pada berbagai derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum. Metode : Penelitian ini bersifat deskriptif analitik dengan pendekatan penelitian cross sectional. Penelitian ini dilakukan dengan pengambilan dan pengumpulan blok parafin jaringan ovarium sebanyak 45 sampel terdiri dari 22 sampel adenokarsinoma serosum dan 13 sampel adenokarsinoma musinosum, dipulas dengan imunohistokimia PGP9.5 dan dilakukan interpretasi dengan menilai intensitas kualitas tampilan warna pada sel tumor. Hasil: Berdasarkan analisa dengan uji Kruskal-Wallis didapati 26 sampel yang terpulas negatif dengan imunohistokimia PGP9.5 terdapat 13 kasus dengan derajat differensiasi well differentiated (50%), 6 kasus (23,1%) moderately differentiated dan 7 kasus (26,9%) poorly differentiated. Pada 5 sampel yang terekspresi lemah tedapat 4 kasus (80%) dengan well differentiated dan 1 kasus (20%) dengan poorly differentiated. Pada 4 kasus terekspresi sedang, terdapat 2 kasus (50%) pada well differentiated, 1 kasus (25%) pada moderately differentiated dan 1 kasus (25%) pada poorly differentiated. Pada 10 sampel yang terekspresi kuat, terdapat 2 kasus(20%) pada well differentiated dan 8 kasus (80%) pada poorly differentiated Kesimpulan: Tidak ada hubungan ekspresi PGP9.5 dengan derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum (p-value > 0.05).