| dc.description.abstract | Heavy metal pollution is a problem and lead pollution in particular represents a serious problem in develop nations and also developing nations like Indonesia. Lead contamination especially in humans, and animals occurs by polluted air, water, and land. Lead pollution is especially heavy in motor vehicle exhaoust and certain industries, as well as a consequence of inadequate disposal of lead batteries. Lead can accumulate in body tissues and damage the structure of lipid cell membranes. This damage can be measured by the rate of formation of malondialdehyde (MDA), a product of lipid peroxidation. The goal of this research was to know the influence of lead acetate exposure and vitamin C on the rate of MDA formation and the quality of spermatozoa in mice, as measured in secretions from the caudal epididymis. This experimental research was conducted using 36 male mice ( Mus musculus L) albino Strain Balb/c that were into six treatment groups each consisting of six mice. The lead acetate and vitamin C were administered orally over 36 days. The results of this research showed a significant increase (p | en_US |
| dc.description.abstract | Heavy metal pollution is a problem and lead pollution in particular represents a serious problem in develop nations and also developing nations like Indonesia. Lead contamination especially in humans, and animals occurs by polluted air, water, and land. Lead pollution is especially heavy in motor vehicle exhaoust and certain industries, as well as a consequence of inadequate disposal of lead batteries. Lead can accumulate in body tissues and damage the structure of lipid cell membranes. This damage can be measured by the rate of formation of malondialdehyde (MDA), a product of lipid peroxidation. The goal of this research was to know the influence of lead acetate exposure and vitamin C on the rate of MDA formation and the quality of spermatozoa in mice, as measured in secretions from the caudal epididymis. This experimental research was conducted using 36 male mice ( Mus musculus L) albino Strain Balb/c that were into six treatment groups each consisting of six mice. The lead acetate and vitamin C were administered orally over 36 days. The results of this research showed a significant increase (p en_US
dc.description.abstract Masalah polusi logam berat termasuk salah satunya timbal merupakan masalah yang serius di negara-negara maju maupun berkembang seperti Indonesia. Timbal dapat terkontaminasi terutama pada manusia, dan hewan melalui udara, air, dan tanah. Polusi timbal terutama berasal dari gas buang kendaraan bermotor dan cemaran limbah industri baterai aki. Sifatnya yang terakumulasi di dalam jaringan tubuh berdampak terhadap kerusakan struktur lipid membran sel, ditandai dengan meningkatnya kadar malondialdehyde (MDA) sebagai salah satu produk dari peroksidasi lipid. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemaparan timbal asetat dan vitamin C yang diberikan bersamaan secara oral selama 36 hari terhadap kadar malondialdehyde dan kualitas spermatozoa di dalam sekresi cauda epididimis mencit. Penelitian ini dilakukan secara eksperimental, 36 ekor mencit (Mus musculus L) albino jantan Strain Balb/c dibagi menjadi 6 kelompok perlakuan yang masing-masing terdiri dari 6 ekor. Hasil penelitian menunjukkan terjadi peningkatan kadar MDA bermakna (p<0,05) pada kelompok perlakuan timbal asetat 0,1 % w/v, dan timbal asetat 0,3 % w/v dibandingkan dengan kelompok perlakuan aquadest/kontrol. Penambahan vitamin C dengan dosis 0,2 mg/g BB pada mencit yang dipapar timbal asetat 0,1% w/v dapat menurunkan kadar malondialdehyde secera bermakna (p<0.05) dibandingkan dengan kelompok yang diberikan tanpa penambahan vitamin C. Pemberian vitamin C dosis 0,2 mg/g BB kurang bekerja efektif menurunkan kadar malondialdehyde (p>0,05) jika diberikan pada kelompok mencit yang dipapar timbal asetat 0,3 % w/v dibandingkan dengan kelompok tanpa penambahan vitamin C. Pemberian vitamin C saja tidak berpengaruh bermakna (p>0.05) terhadap kadar malondialdehyde dibandingkan dengan kelompok perlakuan aquadest/kontrol. Pemberian timbal asetat 0,1 % w/v atau 0,3 % w/v pada mencit dapat mempengaruhi penurunan jumlah, motilitas, kecepatan gerak, dan persentase morfologi normal spermatozoa secara bermakna (p<0,05) bila dibandingkan dengan kelompok perlakuan aquadest/kontrol. Penambahan vitamin C 0,2 mg/g BB dapat meningkatkan motilitas, dan kecepatan gerak secara bermakna (p<0,05) pada hewan uji yang dipapar timbal asetat 0,1 % w/v dibandingkan dengan kelompok hewan uji tanpa penambahan vitamin C. Hasil lainnya menunjukkan pemberian vitamin C saja pada hewan uji ternyata tidak berpengaruh nyata (p>0,05) terhadap penurunan kualitas spermatozoa. | en_US |
