Ekspresi Imunohistokimia Aromatase P450 pada Endometrium Ektopik Penderita Endometriosis Dibandingkan Endometrium Normal

Abstract

OBJECTIVE : To describe the differences of aromatase P450 expression by imunohistochemistry preparation of ectopic endometrium endometriosis patients compared with normal endometrium. METHODS : This study is a cross- sectional study to 32 paraffins blocks of ectopic endometrial tissue obtained from patients with endometriosis of laparotomy and laparoscopy, while control is 32 endometrial paraffins of post-hysterectomy due to non estrogen-dependent diseases. Paraffin tissue contained in the managed and performed immunohistochemical staining using the placenta at term as a positive control . These results were interpreted based on the strength of the color intensity and statistically analyzed. RESULTS : Of the 32 cases of endometriosis were observed, there were 30 (97%) of ectopic endometrium of endometriosis patients with intensity +3 , and not found at all ectopic endometrium of patients with endometriosis with +1 and negative intensity , while 22 cases (68.8 %) of the 32 cases of normal endometrium were intensity +1 , and no cases with intensity +3. P450 aromatase expression in endometriosis is higher than the normal endometrium, and this difference was statistically significant ( < 0.05 ) In relation to the degree of endometriosis with the intensity of IHC, 17 cases ( 100 % ) patients with endometriosis stage 4 were +3 stain intensity, but there was no difference among the degree of endometriosis with aromatase P450 expression ( P>0.05 ) CONCLUSION : The P450 aromatase expression in ectopic endometrium of patients endometriosis where higher than normal endometrium , although there was no correlation with the degree of endometriosis aromatase expression .

TUJUAN: Penelitian ini bertujuan untuk mengetahui perbedaan ekspresi aromatase P450 pada endometrium ektopik (endometriosis) dibandingkan endometrium normal. METODE PENELITIAN: Penelitian ini bersifat cross sectional terhadap 32 parafin blok jaringan endometrium ektopik penderita endometriosis yang diperoleh dari hasil laparatomi dan laparaskopi, sedangnkan kontrol diambil dari 32 endometrium kasus post histerektomi atas indikasi penyakit tidak tergantung estrogen. Jaringan yang terdapat dalam parafin tersebut dikelola dan dilakukan pewarnaan imunohistokimia dengan menggunakan plasenta aterm sebagai kontrol positif. Hasil tersebut diinterpretasi berdasarkan kekuatan intensitas warna dan dianalisa secara statistik. HASIL: Dari 32 kasus endometriosis yang diamati, sebanyak 30 (97%) sediaan endometrium ektopik penderita endometriosis terwaranai +3, dan tidak ditemukan sama sekali endometrium ektopik penderita endometriosis dengan intensitas +1 dan negatif, sedangkan 22 kasus (68,8%) dari 32 kasus endometrium normal intensitas +1, dan tidak ada yang intensitas +3. Ekspresi aromatase P450 pada endometriosis lebih tinggi dibandingkan endometrium normal, dan perbedaan ini bermakna secara statistik (<0,05) Pada kaitan derajat endometriosis dengan intensitas IHC, sebanyak 17 kasus (100%) penderita endometriosis stadium 4 terwarnai intensitas +3, namun tidak terdapat hubungan derajat endometriosis dengan ekspresi aromatase P450 (P>0,05) KESIMPULAN: Terdapat perbedaan ekspresi aromatase P450 pada endometrium ektopik penderita endometriosis dibandingkan endometrium normal, walaupun tidak terdapat hubungan ekspresi aromatase dengan derajat endometriosis.