Perbedaan Aktivitas Antibakteri Flavonoid Total dan Tanin Total Albedo Buah Jeruk Bali (Citrus Maxima Merr.)

Abstract

Albedo of bali citrus fruit powder extracted with ethanol 96% by maceration then maserat obtained evaporated using a rotary evaporator. Further concentrated methanol extract was dissolved with ethyl acetate to obtain a total tannins. Concentrated ethyl acetate extract was partitioned with n-hexane until clear and concentrated methanol layer in the water bath to obtain a concentrated methanol extracts are flavonoids total. Furthermore, total flavonoids and tannins total tested its activity against Escherichia coli, Shigella dysenteriae and Salmonella typhi. Results of the determination of total flavonoid concentration of 0.185% and a total of 9.05% tannin were both dark blue. Total flavonoids are more effective as an antibacterial against Shigella dysenteriae bacteria with the highest inhibitory zone diameter of 19.1 mm, while for the bacteria Salmonella typhi and Escherichia coli with a diameter of 17.4 mm and 15.4 mm at a concentration of 25 ppm. While the total tannins to the bacteria Salmonella typhi, Shigella dysenteriae and Escherichia coli respectively inhibitory diameter of 15.7; 14.6 and 10.6 mm at a concentration of 25 ppm.

Serbuk albedo buah jeruk bali (Citrus maximaMerr.) diekstraksi dengan pelarut metanol 96% secara maserasi kemudian maserat yang diperoleh diuapkan menggunakan rotary evaporator. Selanjutnya ekstrak metanol pekat dilarutkan dengan etil asetat untuk memperoleh tanin total. Ekstrak pekat etil asetat dipartisi dengan n-heksan sampai bening dan dipekatkan di penangas water bath sampai diperoleh ekstrak pekat metanol yaitu flavonoid total. Selanjutnya flavonoid total dan tanin total diuji aktivitasnya terhadap bakteri Escherichia coli, Shigella dysenteriae dan Salmonella typhi. Hasil penentuan kadar flavonoid total sebesar 0,185% dan tanin total sebesar 9,05% yang keduanya berwarna biru gelap. Flavonoid total lebih efektif sebagai anti bakteri yaitu terhadap bakteri Shigella dysenteriae dengan diameter zona hambat tertinggi yaitu 19,1 mm sedangkan untuk bakteri Salmonella typhi dan Escherichia coli dengan diameter 17,4 mm dan 15,4 mm pada konsentrasi 25 ppm. Sedangkan pada tanin total terhadap bakteri Salmonella typhi, Shigella dysenteriae dan Escherichia coli masing-masing diameter hambatnya sebesar 15,7; 14,6 dan 10,6 mm pada konsentrasi 25 ppm.