Ekspresi Reseptor Leptin pada Jaringan Endometriosis

Abstract

Introduction: The pathogenesis in endometriosis widely studied in the hope of finding a new marker for diagnostic or therapeutic. Recently leptin and its receptor is said to be involved in regulating the production of hormones, ovulation, physiology, implantation, angiogenesis, and immune homeostasis in endometriosis. However, there is still few researches that determine leptin receptor expression in endometriosis. Objective: determine difference of receptor leptin expression between endometriosis and normal endometrial tissue. Methods: This study is an analytic study in 23 cases of paraffin blocks of ectopic endometrial tissue and 23 paraffin blocks of normal endometrial tissue. Demographic data such as age, parity, and stage endometriosis obtained from medical records. Leptin receptor expression was analyzed by immunohistochemistry examination based leptin receptor antibody staining. Perfomed interpretation performed by two people pathologist. Said to be negative when no cells are stained, +1 if 10-50% of the cells were stained, +2 if> 50% of cells stained weakly, +3 nine> 50% of cells stained strongly. Data analyzed by Chi-square test or Fisher exact. The limit of significance in this study was 95% (p <0.05). Result: Majority of endometriosis patients aged under 30 years (47.8%), all nullipara (100%), and at stage 3 (43.5%). Expression of leptin receptor entirely +3 (100%), while majority in endometrium group showed negative expression (43.5%). Fischer's exact test results indicate that there was significant difference of leptin receptor expression between endometriosis and normal endometrial tissue (p=0.001). Conclusion: There was significant difference of leptin receptor expression between endometriosis and normal endometrial tissue

Pendahuluan: Patogenesis defek pada endometriosis banyak dipelajari dengan harapan dapat menjadi penanda diagnositk atau pengembangan terapi target. Baru-baru ini leptin dan reseptornya ditemukan terlibat dalam pengaturan produksi hormon, ovulasi, fisiologi, implantasi, angiogenesis, dan homeostasis imun endometriosis. Namun, masih sedikit penelitian yang membandingkan bagaimana ekspresi reseptor leptin pada jaringan endometrium normal dengan jaringan endometriosis. Tujuan: Untuk mengetahui perbedaan ekspresi reseptor leptin pada jaringan endometriosis dibandingkan endometrium normal. Metode: Penelitian ini adalah penelitian analitik pada 23 parafin blok jaringan endometriosis dan 23 blok jaringan endometrium normal. Data demogradi seperti usia, paritas, dan stadium endeomtriosis diperoleh dari rekam medik. Ekspresi reseptor leptin dianalisis dengan pemeriksaan imunohistokimia yang dikatakan negatif bila tidak ada sel yang terwarnai, +1 bila 10-50% sel yang terwarnai, +2 bila >50% sel terwarnai lemah, +3 bila >50% sel terwarnai kuat. Data hasil penelitian ini dianalisis dengan uji Fisher exact dnegan batas kemaknaan p<0,05. Hasil: Mayoritas pasien endometriosis berusia di bawah 30 tahun (47,8%), seluruhnya nullipara (100%), dan berada pada stadium 3 (43,5%). Kelompok endometriosis seluruhnya menunjukkan ekspresi leptin +3 (100%) sedangkan pada kelompok endometrium normal lebih banyak dengan ekspresi negatif (43,5%). Hasil uji Fischer exact menunjukkan adanya perbedaan nilai ekspresi reseptor leptin yang bermakna antara jaringan endometriosis dibandingkan dengan jaringan endometrium normal (p=0,001). Kesimpulan: Ada perbedaan nilai ekspresi reseptor leptin yang bermakna antara jaringan endometriosis dibandingkan dengan jaringan endometrium normal.