Imunoreaktivitas Neuregulin I Serum dan SNP8NRG433E1006 Gen Neuregulin I Pada Suku Batak yang Menderita Skizofrenia Paranoid

Abstract

Background Neuregulin 1 (NRG1) gene is located at 8p13, one of the best replicated linkage loci for schizophrenia. NRG1 is a pleiotropic growth factor, important in nervous system development and function. It is involved in the modulation of neuronal migration, synaptogenesis, gliogenesis, neuron-glia communication, myelination and neurotransmission in the brain and other tissues. SNP8NRG433E1006 gene NRG1 is one of the five high risk SNP in schizophrenia. Objective to look for differences NRG1 immunoreactivity serum and SNP8NRG433E1006 NRG1 gene in Bataks ethnic with schizophrenia paranoid and without mental disorders. Methods This study was approved by the Research Ethics Committee of Medical Faculty University of Sumatera Utara. Written informed consents were obtained from all participant after giving a full explanation of the study protocol. Semi-structured interviews using MINI-ICD X were carried out for all participants. Diagnosis of schizophrenia paranoid were made based on PPDGJI-III criteria. DNA extraction, Nested PCR, DNA sequencing and ELISA were done for participants. Results Mean NRG1-immunoreactivity serum in Batak ethnic with schizophrenia paranoid 14,51+6,81pg/ml, without mental disorder 13,12 +2,49 pg/ml. There was statistically significant difference between mean serum-NRG1-immunoreactivity in Batak ethnic with schizophrenia paranoid and without mental disorder (p=0,036 ). There was no statistically significant difference between mean serum-NRG1-immunoreactivity in Bataks men with schizophrenia paranoid and without mental disorder (p=0,574). There was statistically significant difference between mean serum-NRG1-immunoreactivity in Bataks women with schizophrenia paranoid and without mental disorder (p=0,012). There were no statistically significant difference among age, duration of illness, onset of illness, dose of antipsychotic medication and serum-NRG1-immunoreactivity, and the correlation was very weak (r between 0,00-0,20). There was G/A allele polymorphism in 76bp, G/T allele polymorphism in 112bp, and deletion in 110bp. In Batak ethnics, there was a sequence difference in 113-116bp, and Batak ethnics with ATCG sequence in 113-116bp gave higher chance for having schizophrenia then Batak ethnics with GATC sequence in 113-116bp (OR: 0,125; p<0,05; 95%CI 0,04-0,39).

Background Neuregulin 1 (NRG1) gene is located at 8p13, one of the best replicated linkage loci for schizophrenia. NRG1 is a pleiotropic growth factor, important in nervous system development and function. It is involved in the modulation of neuronal migration, synaptogenesis, gliogenesis, neuron-glia communication, myelination and neurotransmission in the brain and other tissues. SNP8NRG433E1006 gene NRG1 is one of the five high risk SNP in schizophrenia. Objective to look for differences NRG1 immunoreactivity serum and SNP8NRG433E1006 NRG1 gene in Bataks ethnic with schizophrenia paranoid and without mental disorders. Methods This study was approved by the Research Ethics Committee of Medical Faculty University of Sumatera Utara. Written informed consents were obtained from all participant after giving a full explanation of the study protocol. Semi-structured interviews using MINI-ICD X were carried out for all participants. Diagnosis of schizophrenia paranoid were made based on PPDGJI-III criteria. DNA extraction, Nested PCR, DNA sequencing and ELISA were done for participants. Results Mean NRG1-immunoreactivity serum in Batak ethnic with schizophrenia paranoid 14,51+6,81pg/ml, without mental disorder 13,12 +2,49 pg/ml. There was statistically significant difference between mean serum-NRG1-immunoreactivity in Batak ethnic with schizophrenia paranoid and without mental disorder (p=0,036 ). There was no statistically significant difference between mean serum-NRG1-immunoreactivity in Bataks men with schizophrenia paranoid and without mental disorder (p=0,574). There was statistically significant difference between mean serum-NRG1-immunoreactivity in Bataks women with schizophrenia paranoid and without mental disorder (p=0,012). There were no statistically significant difference among age, duration of illness, onset of illness, dose of antipsychotic medication and serum-NRG1-immunoreactivity, and the correlation was very weak (r between 0,00-0,20). There was G/A allele polymorphism in 76bp, G/T allele polymorphism in 112bp, and deletion in 110bp. In Batak ethnics, there was a sequence difference in 113-116bp, and Batak ethnics with ATCG sequence in 113-116bp gave higher chance for having schizophrenia then Batak ethnics with GATC sequence in 113-116bp (OR: 0,125; p<0,05; 95%CI 0,04-0,39).