Pengaruh Ekstrak Etanol Buah Labu Siam(Sechium Edule Jacq.Swartz . ) terhadap Aktivitas Glutathione Peroksidasepada Mencit Hiperglikemia yang Diinduksi Streptozotocin (STZ)

Abstract

Streptozotocin causing hyperglycemia in animal models through oxidative stress mechanism that damage the β cells pancreatic. The ethanol extract of Sechium edule can reduce oxidative stress. The purpose of this study was to determine the effects EEBLS to decrease blood sugar levels, increased activity of the enzyme glutathione peroxidase and influence on pancreatic histological white male mice induced by STZ. This research is experimental research design post test randomized controlled group design, using male white mice (Mus musculus L.) Strains DD Webster were randomized into 4 groups: negative control group, positive control group, the group receiving EEBLS 100 mg / kg , and the group that received EEBLS 200 mg / kg. The results showed a decrease in blood sugar levels significantly when compared with the control group. Glutathione peroxidase enzyme activity in this study, but no significant decrease when compared with the control group. Histological observation of the pancreas showed a change in diameter in the group receiving EEBLS. The conclusion of this study is EEBLS 200 mg / kg significantly lowered blood sugar levels in mice, there was no change in the activity of the enzyme glutathione peroxidase significant with EEBLS administration and there are differences in the diameter of the pancreatic β cells significantly in the group given EEBLS 200 mg / kg. The purpose of this study was to determine the effect EEBLS male white mice induced by STZ. This study used male white mice (Mus musculus L.) Strains DD Webster were randomized into 4 groups by using post test study design randomized controlled group. It was found that the ethanol extract of Sechium edule 200 mg/kgbb may decreased blood glucose levels and pancreatic β cell diameter greater than the control group, but there were no significant differences in the enzyme glutathione peroxidase. Sechium edule antioxidant effects caused contain flavonoids.

Streptozotocin menyebabkan hiperglikemia pada hewan coba melalui mekanisme stress oksidatif yang merusak sel β pankreas. Pemberiaan ekstrak etanol buah labu siam dapat menurunkan stress oksidatif. Tujuan penelitian ini adalah untuk mengetahui efek EEBLS terhadap penurunan kadar gula darah, peningkatan aktivitas enzim glutatione peroksidase dan pengaruh terhadap gambaran histologis pankreas mencit putih jantan yang diinduksi STZ. Penelitian ini merupakan penelitian eksperimental dengan rancangan penelitian post test randomized controlled group design, menggunakan mencit putih jantan (Mus musculus L.) Strain DD Webster yang dirandomisasi dalam 4 kelompok yakni kelompok kontrol negatif, kelompok kontrol positip, kelompok yang mendapatkan EEBLS 100 mg/kgBB, dan kelompok yang mendapat EEBLS 200 mg/kgBB. Hasil penelitian menunjukkan adanya penurunan kadar gula darah yang bermakna bila dibandingkan dengan kelompok kontrol. Aktivitas enzim glutation peroksidase pada penelitian ini terjadi penurunan namun tidak signifikan bila dibandingkan dengan kelompok kontrol. Pengamatan terhadap histologis pankreas menunjukkan adanya perubahan diameter pada kelompok yang mendapatkan EEBLS. Kesimpulan penelitian ini adalah EEBLS 200 mg/kgbb secara signifikan menurunkan kadar gula darah mencit, tidak ada perubahan aktivitas enzim glutatione peroksidase yang signifikan dengan pemberian EEBLS dan terdapat perbedaan diameter sel sel β pankreas yang signifikan pada kelompok yang diberi EEBLS 200 mg/kgbb.