Hubungan Ekspresi Imunohistokimia Mucin 1 (MUC1) dengan Grading Histopatologi dan Subtipe Molekuler Pada Karsinoma Payudara Invasif

Abstract

Breast carcinoma is a malignancy that occurs from breast cells that grow and continue to proliferate uncontrollably. Mucin 1 (MUC1) has become a topic of interest in cancer treatment due to its upregulation, which affects the invasion, proliferation, and survival of tumor cells, by reducing cells adhesion and extracellular cell-matrix adhesion. Objective: To analyze the correlation between the immunohistochemical expression of Mucin 1 (MUC1) with histopathological grading and molecular subtypes in invasive breast carcinoma. Methods: Formalin-fixed tissue paraffin blocks from 42 patients with invasive breast carcinoma were used as research samples and Mucin 1 (MUC1) immunohistochemical staining was assessed. The correlation between Mucin 1 (MUC1) expression and histopathological grading and molecular subtypes was analyzed by statistical tests using the SPSS program. Results: Of the 42 samples studied, the most histopathological subtypes were invasive carcinoma of no special type (85.7%), with grade 2 being the most common (42.8%). The most common molecular subtype was luminal B (40.5%). There were four molecular subtypes in invasive breast carcinoma of no special type, and the most common was HER2 enriched with 14 samples (39.0%). While invasive lobular carcinoma was found in luminal B (100%), mucinous carcinoma was found in luminal A (100%). Mucin 1 (MUC1) was expressed in 73.8% of invasive breast carcinoma specimens. Conclusion: There is a significant correlation between the immunohistochemical expression of Mucin 1 (MUC1), either with histopathological grading or with molecular subtypes in invasive breast carcinoma (p-value were 0.027 and 0.010, respectively)

Karsinoma payudara merupakan keganasan yang terjadi dari sel-sel payudara yang tumbuh dan terus berproliferasi dengan tidak terkendali. Mucin 1 (MUC1) telah menjadi topik perhatian dalam pengobatan kanker dikarenakan upregulation, yang mempengaruhi invasi, proliferasi dan kelangsungan hidup sel tumor, dengan mengurangi adhesi sel-sel dan adhesi matriks sel ekstraseluler. Tujuan: Menganalisis hubungan ekspresi imunohistokimia Mucin 1 (MUC1) dengan grading histopatologi dan subtipe molekuler pada karsinoma payudara invasif. Metode: Blok parafin jaringan yang terfiksasi formalin dari 42 penderita karsinoma payudara invasif digunakan sebagai sampel penelitian dan dilakukan penilaian pewarnaan imunohiskimia Mucin 1 (MUC1). Hubungan ekspresi Mucin 1 (MUC1) dan grading histopatologi dan subtipe molekuler dianalisis dengan uji statistik menggunakan program SPSS. Hasil: Dari 42 sampel yang diteliti dijumpai subtipe histopatologi yang paling banyak adalah invasive carcinoma of no special type (85,7%), dengan grade 2 yang paling banyak dijumpai (42,8%). Untuk subtipe molekuler yang paling banyak dijumpai adalah luminal B (40,5%). Tampak dijumpai keempat subtipe molekuler pada invasive breast carcinoma of no special type, dan yang paling banyak dijumpai adalah HER2 enriched sebanyak 14 sampel (39,0%). Sementara invasive lobular carcinoma dijumpai luminal B (100%), mucinous carcinoma dijumpai luminal A (100%). Mucin 1 (MUC1) terekspresi pada 73,8% spesimen karsinoma payudara invasif. Simpulan: Terdapat hubungan yang signifikan antara ekspresi imunohistokimia Mucin 1 (MUC1), baik dengan grading histopatologi, ataupun dengan subtipe molekuler pada karsinoma payudara invasif (p- value masing-masing adalah 0,027 dan 0,010).