| dc.description.abstract | Tumbuhan inggir-inggir (Solanum sanitwongsei Craib.) dari keluarga
Solanaceae yang dikenal dengan nama terung Siam (Jawa), digunakan sebagai
obat nyeri haid, obat kencing manis, obat tekanan darah tinggi, dan buahnya
digunakan sebagai obat kanker serviks secara turun temurun. Penelitian ini
bertujuan untuk mengetahui efek sitotoksik dari ekstrak dan fraksi buah inggiringgir,
selektivitas indeks, siklus sel, apoptosis dan peningkatan ekspresi protein
p-53 pada sel kanker serviks (hela).
Ekstrak etanol diperoleh secara maserasi dari buah inggir-inggir dengan
pelarut etanol 96%, selanjutnya difraksinasi dengan pelarut n-heksan dan etilasetat
menghasilkan fraksi n-heksan, fraksi etilasetat dan fraksi air (sisa). Ekstrak etanol
buah inggir-inggir, fraksi n-heksan buah inggir-inggir, fraksi etilasetat buah
inggir-inggir dan Fraksi air buah inggir-inggir diuapkan dengan bantuan rotary
evaporator dan dikeringkan dengan freeze dryer. Simplisia dan ekstrak etanol
dilakukan karakterisasi dan skrining fitokimia, sedangkan pada fraksi juga
dilakukan skrining fitokimia. Selanjutnya ekstrak dan fraksi dilakukan uji
sitotoksik terhadap sel hela menggunakan metode MTT (Microculture
Tetrazolium Technique). Fraksi yang paling aktif dilanjutkan untuk uji
selektivitas indeks, mekanisme penghambatan siklus sel, apoptosis dengan metode
flowsitometri dan efek peningkatan ekspresi protein p-53 pada sel hela dengan
metode imunositokimia.
Hasil karakterisasi simplisia dan ekstrak etanol buah inggir-inggir
diperoleh kadar air berturut-turut 7,32%; 4,97%; kadar sari larut air 18,75%;
12,57%; kadar sari larut etanol 15,65%; 59,71%; kadar abu total 3,86%; 0,47%
serta kadar abu yang tidak larut asam 0,43%; 0,08%. Hasil skrining fitokimia
simplisia dan ekstrak diperoleh golongan alkaloid, flavonoid, glikosida, saponin,
tanin dan triterpenoid/ steroid. Skrining fitokimia fraksi n-heksan positif
steroid/triterpenoid, skrining fitokimia fraksi etil asetat positif alkaloid, flavonoid,
glikosida, saponin dan tanin, skrining fitokimia fraksi air positif glikosida dan
saponin. Hasil pengujian sitotoksik larutan uji terhadap sel hela memberikan nilai
IC50 ekstrak etanol buah inggir-inggir sebesar 178,194 μg/ml, fraksi n-heksan
buah inggir-inggir 812,954 μg/ml, fraksi etilasetat buah inggir-inggir 85,489
μg/ml dan fraksi air buah inggir-inggir 241,580 μg/ml. Fraksi etiasetat buah
inggir-inggir merupakan fraksi yang paling aktif dengan nilai Selektivitas indeks
4,05 menunjukkan selektif terhadap sel hela. Fraksi etilasetat buah inggir-inggir
dengan konsentrasi 1X IC50 menghambat siklus sel pada fase G2-M, konsentrasi
2X IC50 menghambat siklus sel pada fase sub G1, sedangkan pengujian apoptosis
menunjukkan mekanisme apoptosis dikarenakan terjadi peningkatan ekspresi
protein p-53 pada sel hela. | en_US |
| dc.description.abstract | Inggir-inggir (Solanum sanitwongsei Craib.) of the family Solanaceae
which is also known as Terung Siam (Java), has been used traditionally for
menstrual pain medication, diabetes, high blood pressure medication, and also
used for curing cervical cancer. The aim of this study was to determine the
cytotoxic effect; the selectivity index, cell cycle inhibition, apoptosis mechanism
and the enhancement of the expression of p-53 protein by of ethanolic extract and
fractions of inggir-inggir on HeLa cells.
The ethanolic extract was prepared by maceration of the inggir-inggir
fruits using 96% ethanol , then fractionated using n-hexane and ethylacetate
solvent. n-hexane fraction, ethyl acetate fraction and aqueous fraction (residue).
All fractions were evaporated on freeze dryer and were subjects to
characterization and phytochemical. Simplicia and were subjects to phytochemical
screening also were done on fractions of inggir-inggir. Furthermore, extracts and
fractions were tested for cytotoxic activity in HeLa cells using MTT (Tetrazolium
microculture Technique) method. The most active fraction was subjected to
selectivity index test, cell cycle inhibition, apoptosis mechanism with
flowcytometric method and the increase in the expression of p-53 in HeLa cells by
immunocytochemistry method was observed.
The characterization results gave water content 7.32% and 4.97%; watersoluble
extract 18.75% and 12.57%; ethanol-soluble extract content 15.65% and
59.71%; total ash content 3.86% and 0.47% and acidic insoluble ash 0.43% and
0.08%. Phytochemical screening of simplex and ethanolic extract showed the
presence of alkaloid; flavonoid; glycoside; saponin; tannin and
steroids/triterpenoids. n-hexane fraction gave possitive results for the presence of
steroids/triterpenoids; ethylacetate fraction was positive for alkaloid; flavonoid;
glycoside; saponin; and tannin. The aqueous fraction was positive for flavonoid;
glycoside and saponin. The cytotoxic activity test of the sample test solution on
HeLa cells gave IC50 value of ethanolic extract 178.194 μg/ml; n-hexane fraction
812.954 μg/ml, ethylacetate fraction was 85.489 μg/ml and aqueous fraction was
241.580 μg/ml. Ethylacetate fraction is the most active fraction with SI value of
4.05, which means that the sample has selective toxicity against HeLa cells. it can
inhibit cell cycle at the G2-M phase with IC50 concentration, while double IC50
concentration, can inhibit cell cycle at sub-G1 phase. Based on apoptosis testing,
it was showed that apoptotic mechanism is increasing the expression of p-53
protein on HeLa cells. | en_US |
