| dc.description.abstract | Background: Lactobacillus acidophilus is one of the strains of lactic acid bacteria that has been widely used as a probiotic. The ability of L. acidophilus to grow in the digestive system can suppress the growth of enteric pathogenic bacteria and improve the balance of microflora in the digestive system so that it can be utilized to maintain a healthy body. L. acidophilus bacteria exhibit short stationary phases and follow rapid cell viability loss, although stored at freezing temperatures. This study aims to determine the viability of bacterial microenkaosulasi to simulation of stomach acid and bile salts.
Objective: To determine the effect of chitosan-alginate in enhancing the viability of the encapsulated L. acidophilus cell to gastric acid simulation.
Purpose: To determine the effect of chitosan-alginate in enhancing the viability of the encapsulated L. acidophilus cell to gastric acid simulation and bile salts.
Methods: The microencapsulation of bacteria was performed by using a chitosan-alginate polymer, the method used was the extrusion method. Bacterial microencapsulation viability tests were performed by incubating microencapsulation in the stimulation of stomach acid pH 1,2 for 120 minutes and bile salts with concentrations of 1% and 5%.
Result: The microencapsulated L. acidophilus ATCC 4356 cell population amounted to 3.9x107 colonies/ml of suspension, microencapsulated using sodium alginate-chitosan polymer 2:0.1%; 2:0.2%; 2:0.3% with a cell density of 1.4x107; 1.6x107; 3.0x107 colonies/g can still be preserved L. acidophilus cells incubated in a gastric pH 1.5 for 120 minutes.
Conclusion: Polymer sodium alginate-chitosan can maintain the cell number of the cell (Free cell) were still able to retain L. acidophilus cells incubated in a gastric pH 1.5 for 120 minutes simulation. Testing of L. acidophilus micro-encapsulated sodium alginate-chitosan also still able to maintain viability of bacteria incubated 1% and 5% bile salt, wherein the concentration of sodium alginate-chitosan 2: 0,1%; 2: 0.2%; 2: 0.3% obtained consecutive cell density of 2.3 x 107; 2.6 x 107; 3.6 x 107 CFU / g for 1% bile salt concentration, and obtained cell density of 2.0 x 107 respectively; 2.5 x 107; 3.6 x 107 CFU / g for 5% bile salt concentration.
Conclusion: The results concluded that L. acidophilus microencapsulation using alginate-chitosan polymer is still able to maintain L. acidophilus cells incubated in simulated fluid of gastric and bile salts. | en_US |
| dc.description.abstract | Latar belakang: Lactobacillus acidophilus merupakan salah satu strain bakteri asam laktat yang telah banyak dimanfaatkan sebagai probiotik. Kemampuan L. acidophilus ATCC 4356 untuk tumbuh di dalam sistem pencernaan dapat menekan pertumbuhan bakteri patogen enterik dan memperbaiki keseimbangan mikrobioma dalam sistem pencernaan sehingga dapat dimanfaatkan untuk menjaga kesehatan tubuh. Bakteri L. acidophilus menunjukkan fase stasioner yang pendek serta diikuti kehilangan viabilitas sel yang cepat, walaupun disimpan pada suhu beku. Penelitian ini bertujuan untuk mengetahui viabilitas mikroenkapsulasi bakteri terhadap simulasi cairan asam lambung dan garam empedu.
Tujuan: Untuk mnegetahui pengaruh alginat-kitosan dalam meningkatkan viabilitas sel L. acidophilus yang telah terenkapsulasi yang terhadap simulasi asam lambung dan garam empedu.
Metode: Pembuatan mikroenkapsulasi bakteri dilakukan dengan menggunakan polimer alginat-kitosan, sedangkan metode yang digunakan adalah metode ekstrusi. Uji viabilitas mikroenkapsulasi bakteri dilakukan dengan menginkubasi mikroenkapsulasi dalam simulasi cairan asam lambung pH 1,2 selama 120 menit dan garam empedu dengan konsentrasi 1% dan 5%.
Hasil: Hasil mikroenkapsulasi L. acidophilus ATCC 4356 diperoleh jumlah populasi sel sebanyak 3,9 x 107 koloni/ml pada suspensi. Mikroenkapsulasi menggunakan polimer natrium alginat-kitosan konsentrasi 2:0,1% ; 2:0,2% ; 2:0,3% masih mampu mempertahankan sel L. acidophilus yang diinkubasi dalam simulasi cairan lambung pH 1,5 selama 120 menit, dimana diperoleh kepadatan sel berturut-turut sebanyak 1,4 x 107 ; 1,6 x 107 ; 3,0 x 107 CFU/g. Pengujian L. acidophilus yang dimikroenkapsulasi natrium alginat-kitosan juga masih mampu mempertahankan viabilitas bakteri yang diinkubasi garam empedu 1% dan 5%, dimana pada konsentrasi natrium alginat-kitosan 2:0,1% ; 2:0,2% ; 2:0,3% diperoleh kepadatan sel berturut-turut sebanyak 2,3 x 107 ; 2,6 x 107 ; 3,6 x 107 CFU/g untuk konsentrasi garam empedu 1%, dan diperoleh kepadatan sel berturut-turut sebanyak 2,0 x 107 ; 2,5 x 107 ; 3,6 x 107 CFU/g untuk konsentrasi garam empedu 5%.
Kesimpulan: The results concluded that L. acidophilus microencapsulation using alginate-chitosan polymer is still able to maintain L. acidophilus cells incubated in simulated fluid of gastric and bile salts. | en_US |
