| dc.description.abstract | Exposure to UVB rays absorbed by the skin can cause free radicals, namely
Reactive Oxygen Species (ROS), which cause damage to cell components such as
cell walls, membrane lipids, mitochondria, and DNA. Red algae contain
polyphenol and flavonoid compounds that have the potential as antioxidants by
inhibiting oxidation and reducing some of the effects of free radicals. This study
aims to formulate red algae as an anti-aging in nanogel preparations.
This research was conducted experimentally including the manufacture of
ethanolic extract of red algae obtained by soxhletation using ethanol solvent
which was then tested for the content of total phenol and total flavonoid. The
ethanolic extract of red algae was formulated in the form of nanogels with extract
concentrations of 1%, 2%, and 3% and tested for physical characteristics of the
preparation, stability and irritation on the skin. Anti-aging activity in vivo in mice
from the nanogel preparation was then analyzed macroscopically, histologically
and compared with the gel preparation. The rats were irradiated by UVB three
times a week for 10 weeks and the rats were given a red algae extract nanogel
preparation every day, then the rat skin was analyzed macroscopically and
microscopically with hematoxyllin and eosin staining.
The ethanolic extract of red algae contains a total phenol content of 4.3645
mg GAE/g sample and a total flavonoid content of 11.0941 mg QE/g extract. The
resulting red algae ethanol extract nanogel has a thick consistency, orange in color
and was homogeneous and stable after stability testing and did not irritate rabbit
skin. The appearance of the macroscopic results of rat skin that was irradiated by
UVB without the preparation was rough, dry and wrinkled. The group of rats that
were given the preparation after 10 weeks showed a smoother and less wrinkled
skin appearance compared to the group of rats that were given the gel preparation.
Microscopic results of the skin of a group of mice that were irradiated with UVB
without given showed thinning of the epidermis and a reduction in collagen
density. The skin of rat group thar were given the nanogel preparation showed
thicker epidermis changes and increased collagen density.
Based on the results of this study, it can be concluded that the preparation of
red algae extract nanogels has antioxidant activity from the content of polyphenol
and flavonoid compounds. The nanogel preparations were stable for 12 weeks at
room, low, and high temperatures and had better anti-aging activity in vivo than
the gel preparations on rat skin that was irradiated by UVB. | en_US |
