Perbandingan Kadar Reactive Oxygen Species (ROS) Setelah Pemberian Tiamin Intravena dan NaCl 0,9% Intravena pada Pasien yang Menjalani Pembedahan dengan Anestesi Umum

Abstract

Background: Surgery is one of the causes of increased levels of Reactive Oxygen Species (ROS) due to tissue injury that triggers an imbalance between oxidants and antioxidants. Thiamine is an oxidant that is often found to be deficient postoperatively, so it is believed that its administration can suppress oxidative stress resulting from tissue injury during surgery. Aim: To determine the comparison of ROS levels before and after intravenous administration of thiamine and NaCl 0.9% in patients undergoing surgery under general anesthesia. Methods: This study is a clinical trial with a double-blind randomized controlled trial design conducted at the Central Surgical Installation, Emergency Surgery Room, and the post-operative recovery room at HAM Hospital. The study subjects were 18-65 years old patients who had ASA I-II and underwent surgery under general anesthesia. The study subjects were divided into 2 groups, group 1 who received thiamine 100 mg (1 ml) intravenously (iv), and group 2 who received NaCl 0.9% 1 ml iv, where both interventions were given 2 hours before surgery. Blood samples were taken before the intervention and 6 hours after the intervention. Comparative analysis of ROS levels before and after the intervention was analyzed using an independent T-test. Results: This study was followed by 38 study subjects. The baseline ROS level was higher in the NaCl 0.9% group (356.212 ± 96.80 ng/ml) compared to the thiamine group (350.36 ± 116.31 ng/ml) and there was no significant difference between the two groups (p = 0.128). Significant differences in ROS levels were found after the intervention (p = 0.04) with higher ROS levels in the NaCl 0.9% group (363.2074 ± 103.74 vs. 343.239 ± 102.05). Significant differences were also found in ROS levels before and after intervention in the thiamine group (p = 0.02). Conclusion: Significant decrease in ROS levels were found after intravenous administration of thiamine compared to NaCl 0.9% in patients undergoing surgery under general anesthesia.