Aktivitas Antioksidan pada Soyghurt dengan Penambahan Ekstrak Daun Sambung Nyawa (Gynura procumbens) (Soy-Nuraghurt) terhadap Mencit Penderita Kanker

Abstract

This research was conducted to determine the effect of soy-nuraghurt which had been made by soursop extract, sambung-nyawa leaves extract, and stabilizers on mice’s health which had suffered from cancer induced by benzo[a]pyrene. Soy-nuraghurt were analyzed by antioxidant activity, total solids content, water content, ash content, protein content, fat content, crude fiber content, total acid,total lactic acid bacteria, vitamin C content, viscosity, total glucose, total soluble solid and organoleptic tests of color, aroma, flavor and texture. In vivo analysis was using 36 male mice who were divided into six groups, i.e : P0 (control), P1 (benzo[a]pyrene), P2 (extract), P3 (soy-nuraghurt), P4 (benzo[a]pyrene + extract), P5 (benzo[a]pyrene + soy-nuraghurt). In vivo analyzed were weight, histopathology of hepar, and activity of Serum Glutamic Pyruvate Transaminase enzyme. The results showed that the ratio of soursop extract and sambung nyawa leaves extract had highly significant effect on activity antioxidant, ash content, protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, vitamin C content, viscosity, total glucose, the hedonic value of the color, aroma and flavor. Ratio of carboxy methyl cellulose (CMC) and arabic gum had highly significant effect on activity antioxidant, solid content, water content, ash content, protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, vitamin C content, viscosity, total soluble solid, the hedonic value of the aroma, flavor and texture. The interaction between two factors had highly significant effect on protein content, fat content, crude fiber content, total acid, total lactic acid bacteria, viscosity, total glucose and total soluble solid. Ratio of soursop extract and sambung-nyawa leaves of (60%:40%) and ratio of carboxy methyl cellulose (CMC) and arabic gum of (1:2) had used for in vivo analysis (the best product quality). In vivo analysis showed that soynuraghurt benzo[a]pyrene inducted mice could give the preventive effects, decreased and treated the mice’s hepar, decreased and normalized the activity of Serum Glutamic Pyruvate Transaminase enzyme and showed highly significant effect on the mice weight.