Skrining Fitokimia Metabolit Sekunder dalam Daun Binahong (Anredera cordifolia (Ten) Steenis) untuk Uji In Vitro Daya Hambat Pertumbuhan Aeromonas hydrophila

Abstract

Binahong leaves are one of natural product to be used to treat the disease of living organisme due to having secondary metabolite to show antibacterial activity. The purpose of this research was to study secondary metabolite of Binahong leaves of which showing antibacterial, to analysis effectivity of antibacterial Binahong leaves extract to inhibit growth of bacteria Aeromonas hydrophila. An experimental laboratory with six treatments and three replications for in vitro was tested. A paper disc soaked in a solution of Binahong leaves extract was used for in vitro at a concentration of 0% (w/v); 0,2%; 0,4%; 0,6%; 0,8%; positive control (Oxytetracycline), then placed on a Tryptose Soya Agar (TSA) medium containing bacteria A. hydrophila and then incubated at 37oC for 24 hours. In vitro test showed that Binahong leaves extract inhibited the growth of bacteria A. hydrophila with a inhibition area around the paper disc. The increase concentration of Binahong leaves extract enhanced the growth inhibition area. Phytochemical screening of Binahong leaves extract showed that the extracts contained flavonoid, phenol, saponin, alkaloid, triterpenoid and β-sitosterol.