Deteksi dan Analisis Ekspresi Transgen (PhGH) ada Ikan Lele Dumbo (Clarias gariepinus) Transgenik F3

Abstract

Transgenic fish is a species that are changing the genetic structure introduced from other organisms to alter their genetic function in accordance with the introduced gene. One of the things that support the development of transgenic fish strain formation is the ability of individual transgenic transgenes to bequeath to the next generation, so that the genetic trait target genes remain stable in the resulting offspring. This study aims to detect and determine the expression of growth hormone gene (PhGH) on dumbo catfish (Clarias gariepinus) transgenic F3 that resulting from the F2 generation. Transgene detection performed on embryos and larvae of catfish transgenic F3 using PCR with primers ACT 107 and PhGH2. Transgene expression was analyzed by using reverse transcriptase polymerase chain reaction (RT-PCR) with primers β-actin and ACT 107 and PhGH2. The results showed that the transgene was detected in some of the embryos and larvae with a size of 1500 bp fragment. β-actin primer amplification compared to primary ACT 107 and PhGH2 showed that transgene can be expressed in some of the embryos and larvae with a size of 1500 bp fragment.