Ekstrak Daun Sambiloto (Andrographis paniculata) dalam Mengendalikan Pertumbuhan Bakteri Aeromonas hydrophila, Edwarsiella tarda dan Jamur Saprolegnia sp. secara In Vitro

Abstract

This study was aimed to know antimicrobial potential of extract of sambiloto’s Leaf (Andrographis paniculata) against bacteria Aeromonas hydrophila, Edwardsiella tarda and Saprolegnia sp. and to know the extract toxicity to brine shrimp (Artemia salina). Sambiloto’s leaf was extracted using methanol, ethyl acetate and n-Hexane. Antimicrobial activity test was done using diffusion discs method to know the compunds contained in the extract phytochemical test was conducted. The chemical compound test showed that extract of sambiloto’s leaf contained terpenoid/steroid and saponin. The extracts inhibit the growth of Aeromonas hydrophila and Edwardsiella tarda but can not inhibited the growth of Saprolegnia sp. The most optimal extract in inhibiting the bacteria Aeromonas hydrophila and Edwardsiella tarda is extract of ethyl acetate with the average zone of inhibition 9.11 - 10.78 mm in bacteria Aeromonas hydrophila and 6.10 - 9.50 mm in bacteria Edwardsiella tarda. Extract n-hexane has the highest value of LC50 118.6 ppm as classified toxic to Artemia salina. Extracts showed cytotoxic activity with LC50<1000 μg/ml.