Transmisi Transgen (PhGH) dan Performa Pertumbuhan Ikan Lele (Clarias gariepinus) Transgenik F3 di Balai Penelitian Pemuliaan Ikan Sukamandi Subang, Jawa Barat

Abstract

Developments in the field of aquaculture fish production requires the fulfillment of the need to accelerate the pace brood production. Transgenesis technology with the introduction of transgenes (PhGH) can be performed to produce the fish grow faster and have better performance. Transgene detection was performed using the PCR method (Polymerase Chain Reaction) and electrophoresis using transgenic catfish seed F3 progeny. Furthermore, the observation of the performance of catfish transgenic F3 by measuring the degree of fertilization (the number of fertilized eggs / total eggs), hatching (the number of eggs hatched / number of fertilized eggs), the survival rate (the number of early fish / amount of fish end), the performance of growth (weight Average transgenic F3 / weight average non-transgenic) and feed efficiency (absolute growth rate / daily feed intake). Results indicate that the transmission rate ranged from 0-75% with an average transmission in the female parent carriers of the transgene by 2.5% (15/600) and 14.4% (75/520) in the male carriers of the transgene. F3 transgenic catfish have a degree of fertilization 0.37% smaller and has a hatching 4% higher than the non-transgenic. Transgenic catfish feed efficiency higher than non-transgenic This shows transgenic catfish have better performance compared with non-transgenic.