Aktivitas Antimikroba Ekstrak Kulit Buah Manggis (Garcinia mangostana) terhadap Bakteri Aeromonas hydrophila, Streptococcus agalactiae dan Jamur Saprolegnia sp.

Abstract

MADIAH HANDAYANI. Antimicrobial Activity of Extract Mangosteen Rind (Garcinia mangostana) on Aeromonas hydrophila, Edwardsiella tarda Bacteria and Saprolegnia sp. Fungi. Under academic supervision of DWI SURYANTO and TAJUDDIN SIREGAR. This reseach head to determine antimicrobial potential of stem bark extract mangosteen rind (Garcinia mangostana) again bacterial of Aeromonas hydrophila, Edwardsiella tarda and fungus Saprolegnia sp. and determine the level of toxicity bacterial against Artemia Salina Leach. The mangosteen rind was extracted with several solvent, such as methanol, etil asetat and n-hexsana. Testing of antimicrobial activity carried out by disc diffusion method, to determine the compounds contained in the extract phytochemical test. To determine the level of toxicity of the extract using the Brine Shrimp Lethality Test. The chemical compound analysis showed that extract of lotus seed contains fenolik, terpen/steroid dan alkaloid. The extracts inhibitated the growth of Aeromonas hydrophila, Streptococcus agalactiae bacteria and Saprolegnia sp. fungi to some extent. Largest microbial growth inhibition zone of bacteria present in E. tarda by 12 mm.The optimal antimicrobial activity was obtained from etil asetat. The extract showed cytotoxic activity with LC50<1000 μg/ml.