Respon Cylindrocladium sp. terhadap Fungisida Berbahan Aktif Mancozeb secara In Vitro

Abstract

PEBRIAN INDRA RISKY DALIMUNTHE. Response Cylindrocladium sp. To the active substance Mancozeb Fungicide In Vitro. Guided by EDY BATARA MULYA SIREGAR and NELLY ANNA. Cylindrocladium sp. is one of the pathogens trigger dangerous diseases that blight on eucalyptus plants in the pulp industry. Widely spread and prevention with the use of a contact fungicide mancozeb 80% WP. Research on Response Cylindrocladium sp. To the active substanceMancozeb Fungicide In Vitro has been completed. The purpose of this study was to determine the response Cylindrocladium sp. on the provision of different concentrations and characterize the growth of colonies (shape, color, and texture). Research using completely randomized design (CRD) non factorial with basic media PDA. Concentrations used were 0 mg / ml (control) 0.4 mg / ml (M1), 0.8 mg / ml (M2), 1.2 mg / ml (M3), and 1.6 mg / ml (M4). Parameters measured were Cylindrocladium colony diameter sp., The percentage of colonies relative barriers Cylindrocladium sp., Spore density Cylindrocladium sp., Macroscopic and microscopic observation. Treatment significantly affect colony growth, relative barriers and spore density. Macroscopic characteristics include a white colony, the hyphae such as cotton, spread evenly and thicken in the middle. Microscopic characteristics which include changes in the structure of the hyphae, branching hyphae, density klamidiospora, Konia increasingly stunted and swelling of such tumors in a set of hyphae.