| dc.description.abstract | Background: Dragon's blood is a red resin derived from a type of rattan plant
(Daemonorops draco (Willd) Blume; Arecaceae), containing flavonoid
(dracorhodin) and triterpenoid compounds, which have traditionally been used as
wound healing drugs. Jernang resin extract nanoemulgel is a topical preparation
with a small particle size (nano scale) which can accelerate drug absorption,
increase stability and accelerate its pharmacological effect.
Purpose: This study aims to test the antibacterial activity of jernang extract
against Staphylococcus aureus and Pseudomonas aeruginosa bacteria, to test the
safety and stability of the jernang resin extract nanoemulgel and to test its
antibacterial activity and wound healing.
Methods: The extract was made by maceration (96% ethanol solvent) and tested
for its antibacterial activity by the disc diffusion method (Kirby & Bauer test).
Previously, nanoemulsion formulations with surfactants (Tween 80) and
cosurfactants (Isopropyl alcohol) were carried out into 3 formulas with variations
in the concentration of ethanol extract of jernang, namely F1 (2.5%), F2 (5%), and
F3 (7.5%). Furthermore, nanoemulgel was made by mixing nanoemulsion and gel
base with a ratio of 4: 1 with various extract concentrations into 3 formulas,
namely F4 (2%), F5 (4%) and F6 (6%). Preparation of nanoemulsion and
nanoemulgel using high energy emulsification methods, then evaluated physical
stability, antibacterial and wound healing based on percent wound shrinkage and
histology compared with emulgel.
Result: The test results of antibacterial extract at a concentration of 10 mcg / mL
resulted in MIC values of 10.27±0.21 mm (S.aureus) and 9.47±0.15 mm
(P.aeruginosa). Nanoemulgel F4 (2.5%) has good stability during 12 weeks of
storage (temperature variation) organoleptically, pH (5.83), viscosity (76.40
m.Pa.s.), particle size (213.04 nm), stable in the centrifugation test and did not
cause irritation, the MIC values were 11.70 ± 0.10 mm (S.aureus) and 13.50 ±
0.10 mm (P.aeruginosa) while for Gentamicin Sulfate ointment 0.1% was 14.90 ±
1.60 mm and 14.60 ± 0.12 mm, respectively. The results of 100% reduction in
wound diameter were the fastest achieved with treatment for 16 days of 0.1%
Gentamicin Sulfate ointment followed by 18 days of 2% nanoemulgel, 20 days of
4% nanoemulgel, 22 days of 2% emulgel and 4% emulgel for 23 days.
Conclusion: The resin extract of jernang has antibacterial activity. Resin jernang
extract nanoemulgel with a content of 2% (F4) is safe and stable on storage, has
better antibacterial activity, wound healing activity than 2% and 4% jernang
ethanol extract emulgel. | en_US |
