Uji Aktivitas Imunomodulator Ekstrak Daun Mahkota Dewa (Phaleria Macrocarpa) Pada Tikus

Abstract

Cyclophosphamide is an alkylating agent antineoplastic drug which is commonly used to treat cancer. However, the use of cyclophosphamide causes a decrease in the body's immune system, by reducing the proliferation of lymphocytes. Ethyl acetate extract of Mahkota dewa leaves (EEADMD) and ethanol extract of Mahkota dewa leaves (EEDMD) contain flavonoids and saponins which have immunomodulatory effects by acting on lymphokines (interferon ) produced by T cells so that they will stimulate phagocytic cells and increase production. cytokines such as interleukins and interferons. The purpose of this study was to analyze the activity of EEADMD and EEDMD on test animals induced with Staphylococcus aureus by the method of analysis of total leukocytes, differential leukocytes and delayed-type hypersensitivity response. This study used rats which were divided into 2 large groups, namely normal rats without cyclophosphamide induction and rats induced by cyclophosphamide which were then given extract of Dewa's leaves until day 14, on day 4 the test animals were infected with 1% Staphylococcus aureus suspension in the induced group. with cyclophosphamide 70 mg/kg bw performed on days 8 and 13 and then tested for immunomodulatory activity by analysis of total leukocytes, differential leukocytes and delayed-type hypersensitivity response. The results showed that EEDMD 400 mg/kg BW without cyclophosphamide induction and EEDMD 100 mg/kg BW with cyclophosphamide induction could increase total leukocytes and leukocyte differential with a significant difference to the negative group (p < 0.05). The results of the delayed-type hypersensitivity test EEADMD and EEDMD at a dose of 100 mg/kg BW with cyclophosphamide induction and without cyclophosphamide induction could increase the leg volume of rats with a significant difference against the negative group (p < 0.05). This study showed that EEADMD and EEDMD showed an immunomodulatory effect on increasing total leukocytes and leukocyte differential and increasing delayed-type hypersensitivity response