| dc.description.abstract | Cyclophosphamide is an alkylating agent antineoplastic drug which is
commonly used to treat cancer. However, the use of cyclophosphamide causes a
decrease in the body's immune system, by reducing the proliferation of
lymphocytes. Ethyl acetate extract of Mahkota dewa leaves (EEADMD) and
ethanol extract of Mahkota dewa leaves (EEDMD) contain flavonoids and
saponins which have immunomodulatory effects by acting on lymphokines
(interferon ) produced by T cells so that they will stimulate phagocytic cells and
increase production. cytokines such as interleukins and interferons. The purpose
of this study was to analyze the activity of EEADMD and EEDMD on test
animals induced with Staphylococcus aureus by the method of analysis of total
leukocytes, differential leukocytes and delayed-type hypersensitivity response.
This study used rats which were divided into 2 large groups, namely normal
rats without cyclophosphamide induction and rats induced by cyclophosphamide
which were then given extract of Dewa's leaves until day 14, on day 4 the test
animals were infected with 1% Staphylococcus aureus suspension in the induced
group. with cyclophosphamide 70 mg/kg bw performed on days 8 and 13 and then
tested for immunomodulatory activity by analysis of total leukocytes, differential
leukocytes and delayed-type hypersensitivity response.
The results showed that EEDMD 400 mg/kg BW without cyclophosphamide
induction and EEDMD 100 mg/kg BW with cyclophosphamide induction could
increase total leukocytes and leukocyte differential with a significant difference to
the negative group (p < 0.05). The results of the delayed-type hypersensitivity test
EEADMD and EEDMD at a dose of 100 mg/kg BW with cyclophosphamide
induction and without cyclophosphamide induction could increase the leg volume
of rats with a significant difference against the negative group (p < 0.05).
This study showed that EEADMD and EEDMD showed an
immunomodulatory effect on increasing total leukocytes and leukocyte
differential and increasing delayed-type hypersensitivity response | en_US |
