| dc.description.abstract | Hydrogels have elastic, hydrophilic properties and are able to expand and
absorb large amounts of water so that they can be used as good conductors of
active ingredients such as chitosan. Cuttlefish bone is one source of natural
ingredients that contain chitosan. Chitosan is able to act as a wound healer such as
excision cuts. The purpose of this study was to evaluate the effectiveness of
cuttlefish bone chitosan hydrogel on excision wound healing in male white rats.
Chitosan was isolated using acid and alkaline solvent from cuttlefish bone,
then characterized by water content, ash content, protein content, ninhydrin test
and functional groups. The chitosan obtained was made into hydrogel
preparations with a concentration of 0.5% (F1), 1% (F2), 1.5% (F3). Evaluation of
hydrogel preparations included organoleptic, pH, physical characteristics, cross linking, swelling, elasticity, Cycling Test. The effectiveness of the excision
wound was tested on male white rats and measured the diameter of the wound and
the percentage of the excision wound.
The results of the characterization of chitosan have a water content value
of 8.04%, ash content of 1.73% and protein content of 4.8%. The best evaluation
of hydrogel preparations was found in cuttlefish bone chitosan concentration of
0.5% (F1), did not change color, during storage with an average pH value of 4.52.
The results of the characterization of 0.5% chitosan hydrogel (F1) were 8.5 cm in
diameter, 0.45 mm in thickness and >200 times the folding power. 0.5% chitosan
hydrogel (F1) showed 1.3% cross-link, 74.52% swelling, 14.2% elasticity.
Cuttlefish bone chitosan hydrogel 0.5% (F1) can heal rat excision wounds by
increasing the percentage reduction in wound diameter and reduction in wound
diameter. The administration of 0.5% cuttlefish bone chitosan hydrogel gave a
significant difference to the positive control and negative control (p>0.05%).
The conclusion of this study is that 0.5% cuttlefish bone chitosan hydrogel
is the best formula and has the effectiveness of heal excision wounds in male
white rats seen from the reduction in wound diameter and an increase in the
percentage reduction in wound diameter. | en_US |
