Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Gaharu (Aquilaria malaccensis Lamk)

Abstract

SAMUEL FRANSISKUS SILABAN : Phytochemical Screening and Antioxidant Activity Test of Ethanol Extract of Gaharu Leaf (Aquilaria malaccensis Lamk.). Under the supervision of RIDWANTI BATUBARA and HERAWATY GINTING. Gaharu leaf (Aquilaria malaccensis lamk.) today has been popular used by gaharu farmers as a health beverage, but the research of chemical compound that contained in gaharu leaf and the ability of antioxidant activity haven’t yet done. Therefore, a research had been conducted at laboratory farmakognosi, and research lab the faculty of pharmacologists USU in July-October 2013 with extracting the fresh gaharu leaves and simplex by maceration method using a solvent ethanol 96%. Phytochemical screening covering checkings alkaloid contain, glycoside, steroid/triterpenoid, flavonoid, tannin and saponin. The ability testing of activity antioxidant was done with by using DPPH method with the concern parameter is free radicals at the 60th minute with different concentration (40 ppm, 60 ppm, 80 ppm and 100 ppm) and IC50 value (inhibitory concentration) that analyzed by using regression. Inhibitor free radical by extract of fresh gaharu and ethanol simplex showed that high concentration increased the activity of inhibitor free radicals. Test results showed that all of extract and simplex have antioxidant activity. The most active ethanol extract simplex with IC50 value 31,12 ppm and ethanol extract of fresh gaharu leaf 38,16 ppm. The result showed that ethanol extraction of fresh gaharu leaf and simplex contain flavanoid, glycoside, steroid/triterpenoid and tannin as the chemical compounds.