Isolasi dan Potensi Jamur Pelarut Fosfat pada Lahan Bekas Kebakaran Hutan Desa Tongging Kabupaten Karo

Abstract

EVA SERTA P SITORUS: Isolation of Potential Mushroom Phosphates Solvents in the Village Land forest fire Tongging Karo, under the guidance of Dr. DELVIAN SP, MP and Dr. DENI ELFIATI SP, MP. The research was carried out to isolate, examine and identify potential phosphate solubilizing fungi (PSF) to solubilize insoluble phosphate from of the former forest fire Tongging village, Karo. Samples taken from fifty points in a zig zag at 0-20 cm depth around rhizosfir. The chemical analyze properties of the former forest fire soils conducted in North Sumatra Assessment Institute for Agricultural Technology while the activities of isolation, and identification of potential trials conducted at the Laboratory of Soil Biology Agroekoteknologi Studies Program Faculty of Agriculture, University of North Sumatra. Isolation using Pikovskaya media with the source fosfat from Ca3(PO4)2, whereas in the potential test media using Ca3(PO4)2, AlPO4, FePO4 dan Rock Phosphate (RP). as source of insoluble phosphate. Evaluate the potential JPF qualitatively by measuring the diameter of holozone extensive use of the dilution index. JPF potential measurements quantitatively by measuring levels of dissolved phosphate with Bray-II method. The results obtained 7 isolates of isolation of pure PSF. The next test conducted on the potential of isolates obtained. Largest diameter of 2,463cm holozone generated isolates 2 and the smallest diameter of 0,535 cm isolates produced 4. The measurement results are available most of the phosphate levels were 6 isolates of 46,422 ppm (FePO4 ) and the smallest by 1 isolates of 7,875 ppm (FePO4). Based on test results concluded the potential for isolates 1, 2and 6 have the best ability in dissolving phosphate. The results both of macroscopic and microscopically identification showed that isolate number 1, 2 and 6 including of Aspergillus genus.