| dc.description.abstract | Pulmonary tuberculosis is an infectious disease remains a public health
problem in Indonesia and around the world Tuberculosis (J'b) is an infectious
disease caused by rod-shaped bacteria (bacilli), known as Mycobacterium
tuberculosis in most developing countries, the examination of acid1"ast bacilli smear,
culture and radiological examination was used as a method of diagnosis of
tuberculosis in humans. Advanced techniques for the diagnosis of M rapid and
specific tuberculosis is highly emphasized the need for eradication of tuberculosis.
Detection of M tuberculosis by polymerase chain reaction plays an important role
for the diagnosis of smear negative cases, early diagnosis of infection, monitoring
response to treatment, to determine drug resistance in patients and confirm the
certainty of free M tuberculosis. The purpose of this study was to detect M
tuberculosis in patients with pulmonary tuberculosis sputum acid1"ast bacilli staining
(AFB) negative by polymerase chain reaction.
A total of 36 samples of sputum smear-negative pulmonary TB patients
coming from Haji Adam Malik Hospitat General Hospital dr. Pirngadi and Lung
Diseases Clinical Centre Medan examined crosssectional descriptive method.
Detection of M tuberculosis carried out using semi-quantitative PCR techniques
SYBR Green I-based gene targeting IS6I I0 and primers T4 and T5. Sputum samples
decontaminated with NALC-NaOH method is then performed by the method of DNA
extraction spin columns.
From all 3 6 samples of sputum smear-negative pulmonary TB patients from
25 men and 11 women, aged 17-78 years found a positive PCR test results were 13
samples (36.11%) and negative PCR results of 23 samples (63.88%).
PCR can be used to support the diagnosis when the clinical, radiological
support pulmonary Tb with negative sputum smear microscopic, so no need to wait
for culture results for two months. PCR is also an examination of the very useful; fast
and effective anti-TB treatment monitoring in patients and confirm the certainty of
free M tuberculosis. The weakness of the PCR test is able to give positive results
even though there are only 1-10 germs or bacteria in the examination of specimens
dead I dormant and contamination from other specimens. However, in this study has
been carried out of action - preventive action to avoid false positives such as the
arrangement and the sterilization room, the discipline of working procedures,
selection and sterilization of equipment I materials and methods used. | en_US |
